Comparison of platelet ultrastructure and elastic properties in thrombo-embolic ischemic stroke and smoking using atomic force and scanning electron microscopy

Show simple item record Du Plooy, Jeanette Noel Buys, A.V. (Antonia Vergina) Duim, Wiebren Pretorius, Etheresia 2014-02-07T06:32:23Z 2014-02-07T06:32:23Z 2013-07-16
dc.description.abstract Thrombo-embolic ischemic stroke is a serious and debilitating disease, and it remains the second most common cause of death worldwide. Tobacco smoke exposure continues to be responsible for preventable deaths around the world, and is a major risk factor for stroke. Platelets play a fundamental role in clotting, and their pathophysiological functioning is present in smokers and stroke patients, resulting in a pro-thrombotic state. In the current manuscript, atomic force and scanning electron microscopy were used to compare the platelets of smokers, stroke patients and healthy individuals. Results showed that the elastic modulus of stroke platelets is decreased by up to 40%, whereas there is an elasticity decrease of up to 20% in smokers’ platelets. This indicates a biophysical alteration of the platelets. Ultrastructurally, both the stroke patients and smokers’ platelets are more activated than the healthy control group, with prominent cytoskeletal rearrangement involved; but to a more severe extent in the stroke group than in the smokers. Importantly, this is a confirmation of the extent of smoking as risk factor for stroke. We conclude by suggesting that the combined AFM and SEM analyses of platelets might give valuable information about the disease status of patients. Efficacy of treatment regimes on the integrity, cell shape, roughness and health status of platelets may be tracked, as this cell’s health status is crucial in the over-activated coagulation system of conditions like stroke. en_US
dc.description.librarian am2014 en_US
dc.description.librarian ay2014
dc.description.sponsorship National Research Foundation of South Africa for funding of EP. en_US
dc.description.uri en_US
dc.identifier.citation Du Plooy JN, Buys A, Duim W, Pretorius E (2013) Comparison of Platelet Ultrastructure and Elastic Properties in Thrombo-Embolic Ischemic Stroke and Smoking Using Atomic Force and Scanning Electron Microscopy. PLoS ONE 8(7): e69774. DOI: 10.1371/journal.pone.0069774 en_US
dc.identifier.issn 1935-2727
dc.identifier.other 10.1371/journal.pone.0069774
dc.language.iso en en_US
dc.publisher Public Library of Science en_US
dc.rights © 2013 du Plooy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License en_US
dc.subject Thrombo-embolic ischemic stroke en_US
dc.subject Tobacco smoke en_US
dc.subject Platelets en_US
dc.subject Healthy individuals en_US
dc.subject Stroke patients en_US
dc.subject.lcsh Blood platelets -- Examination -- South Africa en
dc.subject.lcsh Cerebrovascular disease -- Patients -- Tobacco use en
dc.subject.lcsh Cerebrovascular disease -- Risk factors en
dc.title Comparison of platelet ultrastructure and elastic properties in thrombo-embolic ischemic stroke and smoking using atomic force and scanning electron microscopy en_US
dc.type Article en_US

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