Rotavirus G and P types circulating in the eastern region of Kenya : predominance of G9 and emergence of G12 genotypes

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dc.contributor.author Kiulia, Nicholas M.
dc.contributor.author Nyaga, Martin M.
dc.contributor.author Seheri, Mapaseka L.
dc.contributor.author Wolfaardt, Marianne
dc.contributor.author Van Zyl, Walda B.
dc.contributor.author Esona, Mathew D.
dc.contributor.author Irimu, Grace
dc.contributor.author Inoti, Murithi
dc.contributor.author Gatinu, Bernard W.
dc.contributor.author Njenga, Peter K.
dc.contributor.author Taylor, Maureen B.
dc.contributor.author Nyachieo, Atunga
dc.date.accessioned 2014-02-03T11:41:36Z
dc.date.available 2014-02-03T11:41:36Z
dc.date.issued 2014
dc.description.abstract BACKGROUND: The World Health Organization has recommended that rotavirus (RV) vaccines be included in all national immunization programs as part of a strategy to control RV-associated diarrheal diseases. Hospital-based surveillance of RV infection is therefore crucial in monitoring the impact pre- and post-vaccine introduction and also to document changes in genotype distribution. This study sought to determine the RV genotypes circulating in the eastern region of Kenya before introduction of the RV vaccine. METHODS: During September 2009 to August 2011, 500 stool samples were collected from children <5 years of age admitted for acute diarrhea in hospitals in the eastern region of Kenya and analyzed for the presence of group A RV using an enzyme immunoassay. G and P genotypes were determined using hemi-nested reverse transcriptase polymerase chain reaction. RESULTS: One hundred and eighty nine out of 500 (38%) samples analyzed were positive for rotavirus. The following G types were detected: G9 (50.9%), G1 (26.8%), G8 (12.1%), G12 (3.1%), G2 (0.6%), mixed G (1.3%) and 5.1% were G nontypeable. P types detected included: P[8] (63.7%), P[4] (12.1%), P[6] (4.5%), mixed P (7.6%) and 12.1% were P nontypeable. The most dominant strain was G9P[8] (35%), followed by G1P[8] (26.8%), G8P[4] (9.6%), G12P[6] (2.5%), G9P[6] (1.9%), G9P[4] (1.3%), G8P[8] (1.3%), and G2P[4] (0.6%). CONCLUSIONS: The present study demonstrates the recurring changing genotypes of RV circulating in Kenya, with genotypes G9, G1 and G8 being the dominant strains circulating in the eastern region of Kenya between 2009 and 2011. Additionally, G12 genotype was detected for the first time in Kenya. en_US
dc.description.librarian hb2014 en_US
dc.description.librarian ay2014
dc.description.sponsorship Investigator-Initiated Studies Program of Merck & Co., Inc., NJ and partial support from the Institute of Primate Research. en_US
dc.description.uri http://www.pidj.com en_US
dc.identifier.citation Kiulia, NM, Nyaga, MM, Seheri, ML, Wolfaardt, M, Van Zyl, WB, Esona, MD, Irimu, G, Inoti, M, Gatinu, BW, Njenga, PK, Taylor, MB &, Nyachieo, A 2014, 'Rotavirus G and P types circulating in the eastern region of Kenya : predominance of G9 and emergence of G12 genotypes', Pediatric Infectious Disease Journal, vol. 33, no.1, Suppl.1, pp. S85-S88. en_US
dc.identifier.issn 0891-3668 (print)
dc.identifier.issn 1532-0987 (online)
dc.identifier.other 10.1097/INF.0000000000000059
dc.identifier.uri http://hdl.handle.net/2263/33222
dc.language.iso en en_US
dc.publisher Lippincott, Williams & Wilkins en_US
dc.rights © 2013 by Lippincott Williams & Wilkins. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en_US
dc.subject World Health Organization en_US
dc.subject Rotavirus G and P types en_US
dc.subject Kenya, Eastern region en_US
dc.subject Rotavirus (RV) en_US
dc.subject.lcsh Rotaviruses -- Kenya en
dc.subject.lcsh Immunization of children -- Kenya en
dc.title Rotavirus G and P types circulating in the eastern region of Kenya : predominance of G9 and emergence of G12 genotypes en_US
dc.type Article en_US


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