This study compares the perceptions of producers and veterinarians on the economicimpacts of Mycobacterium avium subspecies paratuberculosis (MAP) infection in cow-calfherds. Questionnaires were mailed to beef producers through the Designated Johne’sCoordinators and to veterinarians belonging to a nationwide professional organization.Important components of losses associated with MAP infected cows were used to estimatetotal loss per infected cow-year using an iterative approach based on collected survey data.Veterinarians were more likely to perceive a lower calving percentage in MAP infectedcows compared to producers (P = 0.02). Income lost due to the presence of Johne’s disease(JD) in an infected cattle herd was perceived to be higher by veterinarians (P < 0.01). Com-pared to veterinarians without JD certification, seedstock producers were more likely toperceive genetic losses due to culling cows positive for MAP (P < 0.01). There were mixedopinions regarding the magnitude of lowered weaning weight in calves from infected cowsand perceived differences in risk of other diseases or conditions in infected cows. An annualloss of $235 (95% CR: $89–$457) for each infected animal was estimated based on infor-mation from the producer survey. The analogous estimate using information inputs fromveterinarians was $250 ($82–$486). Mean annual loss due to JD in a 100 cow herd with a7% true prevalence was $1644 ($625–$3250) based on information provided by producers.Similarly, mean annual loss based on information collected from veterinarians was $1747($575–$3375).