Isolation and preliminary characterization of a caprine rotavirus

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Date

Authors

Da Costa Mendes, Vasco Manuel
De Beer, M.C.
Goosen, G.H.
Steele, Andrew Duncan

Journal Title

Journal ISSN

Volume Title

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Published by the Agricultural Research Council, Onderstepoort Veterinary Institute

Abstract

Five cytopathic rotavirus strains were isolated in MA 104 cells from stool specimens of kids with diarrhoea. Pre-treatment of the virus with trypsin and incorporation of low levels of trypsin in the maintenance medium were important for the successful cultivation of the strains in these cells. The isolates were shown to be group A rotaviruses by antigenic reactivity with a group A monoclonal antibody. This was confirmed by the migration patterns of the viral RNA genome during polyacrylamide gel electrophoresis, which also confirmed that all five strains had an identical RNA electropherotype. Analysis with monoclonal antibodies to the subgroup-specific VP6 antigen showed that these strains carried the subgroup I epitope.

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Keywords

Veterinary medicine

Sustainable Development Goals

Citation

Da Costa Mendes, VM, De Beer, MC, Goosen, GH & Steele, AD 1994, 'Isolation and preliminary characterization of a caprine rotavirus’, Onderstepoort Journal of Veterinary Research, vol. 61, no. 4, pp. 291-294.