Isolation and preliminary characterization of a caprine rotavirus
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Date
Authors
Da Costa Mendes, Vasco Manuel
De Beer, M.C.
Goosen, G.H.
Steele, Andrew Duncan
Journal Title
Journal ISSN
Volume Title
Publisher
Published by the Agricultural Research Council, Onderstepoort Veterinary Institute
Abstract
Five cytopathic rotavirus strains were isolated in MA 104 cells from stool specimens of kids with diarrhoea.
Pre-treatment of the virus with trypsin and incorporation of low levels of trypsin in the maintenance
medium were important for the successful cultivation of the strains in these cells. The isolates
were shown to be group A rotaviruses by antigenic reactivity with a group A monoclonal antibody.
This was confirmed by the migration patterns of the viral RNA genome during polyacrylamide gel
electrophoresis, which also confirmed that all five strains had an identical RNA electropherotype.
Analysis with monoclonal antibodies to the subgroup-specific VP6 antigen showed that these strains
carried the subgroup I epitope.
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Keywords
Veterinary medicine
Sustainable Development Goals
Citation
Da Costa Mendes, VM, De Beer, MC, Goosen, GH & Steele, AD 1994, 'Isolation and preliminary characterization of a caprine rotavirus’, Onderstepoort Journal of Veterinary Research, vol. 61, no. 4, pp. 291-294.