HIV Type 1 V3 domain serotyping and genotyping in Gauteng, Mpumalanga, KwaZulu-Natal and Western Cape provinces of South Africa

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Engelbrecht, Susan
Smith, Tracey-Lee
Kasper, P.
Faatz, E.
Zeier, Michelle D.
Moodley, D.
Janse van Rensburg, Estrelita

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Mary Ann Liebert

Abstract

More than 20.8 million people are living with HIV/AIDS in sub-Saharan Africa, with southern Africa the worst affected area and accounting for one of the fastest growing AIDS epidemics worldwide. Samples from 81 patients, including 25 from KwaZulu-Natal, 26 from Gauteng, 5 from Mpumalanga, and 25 from Western Cape Province, were serotyped using a competitive V3 peptide enzyme immunoassay (cPEIA). Viral RNA was also isolated from serum and the V3 region amplified by reverse transcriptase polymerase chain reaction (RTPCR) to obtain a 240-bp product for direct sequencing of 29 samples. CLUSTAL W was used to make multiple sequence alignments. Distance calculation, tree construction methods, and bootstrap analysis were done using TREECON. Subtype C-like V3 loop sequences predominate in all provinces tested in South Africa. Discordant sero- and genotype results were observed in one patient only. The correlation between sero- and genotyping was 96% (24 of 25) in KwaZulu-Natal and 100% in Gauteng and Mpumalanga. In Western Cape Province 18% of patients were identified as sero/genotype B and 82% as sero/genotype C. Our data show that results of the second-generation V3 cPEIA correlated well with V3 sequencing and would be a rapid and affordable screening test to monitor the explosive southern African HIV-1 epidemic.

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Sustainable Development Goals

Citation

Engelbrecht, S, Smith, T-L, Kasper, P, Faatz, E, Zeier, M, Moodley, D, Clay, CG & Janse van Rensburg, E 1999, 'HIV Type 1 V3 domain serotyping and genotyping in Gauteng, Mpumalanga, KwaZulu-Natal and Western Cape Provinces of South Africa', AIDS Research and Human Retroviruses, vol. 15, no. 4, pp. 325-328. [http://www.liebertpub.com/publication.aspx?pub_id=2]