Development of a Rift Valley fever real-time RT-PCR assay that can detect all three genome segments

Show simple item record Wilson, William C. Romito, Marco Jasperson, Dane C. Weingartl, Hana Binepal, Yatinder S. Maluleke, Moabi Rachel Wallace, David Brian Jansen van Vuren, Petrus Paweska, Janusz Tadeusz 2013-11-22T10:28:42Z 2013-11-22T10:28:42Z 2013-11
dc.description.abstract Outbreaks of Rift Valley fever in Kenya, Madagascar, Mauritania, and South Africa had devastating effects on livestock and human health. In addition, this disease is a food security issue for endemic countries. There is growing concern for the potential introduction of RVF into non-endemic countries. A number of single-gene target amplification assays have been developed for the rapid detection of RVF viral RNA. This paper describes the development of an improved amplification assay that includes two confirmatory target RNA segments (L and M) and a third target gene, NSs, which is deleted in the Clone 13 commercial vaccine and other candidate vaccines. The assay also contains an exogenous RNA control added during the PCR setup for detection of amplification inhibitors. The assay was evaluated initially with samples from experimentally infected animals, after which clinical veterinary and human samples from endemic countries were tested for further evaluation. The assay has a sensitivity range of 66.7–100% and a specificity of 92.0–100% depending on the comparison. The assay has an overall sensitivity of 92.5%, specificity of 95% and a positive predictive value of 98.7%. The single-tube assay provides confirmation of the presence of RVFV RNA for improved confidence in diagnostic results and a “differentiate infected from vaccinated animals” (DIVA) – compatible marker for RVFV NSs – deleted vaccines, which is useful for RVF endemic countries, but especially important in non-endemic countries. en
dc.description.librarian hb2013 en
dc.description.librarian ab2013
dc.description.sponsorship This project was supported in part by the US Department of Agriculture, Agricultural Research Service Project #58-5430-005-00D through inter-agency agreements with the Science and Technology Directorate of the U.S. Department of Homeland Security under Award Number HSHQDC-07-00982 and the U.S. Department of State Biosecurity Engagement Program. en
dc.description.uri en
dc.identifier.citation Wilson, WC, Romito, M, Jasperson, DC, Weingartl, H, Binepal, YS, Maluleke, MR, Wallace, DB, Van Vuren, PJ & Paweska, JT 2013, 'Development of a Rift Valley fever real-time RT-PCR assay that can detect all three genome segments',Journal of Virological Methods, vol. 193, no. 2, pp. 426-431. en
dc.identifier.issn 0166-0934 (print)
dc.identifier.issn 1879-0984 (online)
dc.identifier.other 10.1016/j.jviromet.2013.07.006
dc.language.iso en en
dc.publisher Elsevier en
dc.relation.requires Adobe Acrobat Reader en
dc.rights © 2013 Elsevier. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Journal of Virological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Virological Methods, vol. 193, no. 2, pp. 426- 431, date. doi : 10.1016/j.jviromet.2013.07.006 This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. en
dc.subject Real-time RT-PCR en
dc.subject RVF en
dc.subject.lcsh Rift Valley fever en
dc.subject.lcsh Zoonoses en
dc.subject.lcsh Polymerase chain reaction en
dc.subject.lcsh Cattle -- Diseases en
dc.subject.lcsh Sheep -- Diseases en
dc.title Development of a Rift Valley fever real-time RT-PCR assay that can detect all three genome segments en
dc.type Article en

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