Transplacental infection in goats experimentally infected with a European strain of bluetongue virus serotype 8

Show simple item record Coetzee, Peter Stokstad, Maria Myrmel, Mette Mutowembwa, Paidamwoyo Loken, Torleiv Venter, Estelle Hildegard Van Vuuren, Moritz 2013-11-22T10:19:55Z 2013-11-22T10:19:55Z 2013-08
dc.description.abstract The capability of the recently emerged European strain of bluetongue virus serotype 8 (BTV-8) to cross the ruminant placenta has been established in experimental and field studies in both sheep and cattle. Seroprevalence rates in goats in North-Western Europe were high during the recent outbreak of BTV- 8; however the capability of the virus to infect goats through the transplacental route has not been established. In the present study, four Saanen goats were inoculated with the European strain of BTV-8 at 62 days of gestation; this resulted in mild clinical signs, however gross lesions observed post mortem were more severe. Viral RNA was detected by real-time RT-PCR in blood and tissue samples from three fetuses harvested from two goats at 43 days post infection. Conventional RT-PCR and genome sequencing targeting viral segment 2 confirmed infection of brain tissue with BTV-8 in two of these fetuses. In total, five of six fetuses demonstrated lesions that may have been associated with transplacental infection with BTV. Infected fetuses did not demonstrate neurological lesions. Low viral RNA concentrations in fetal blood and tissue further suggest that the infected fetuses would probably not have been born viraemic. The implications of these findings with regards to the epidemiology and overwintering of BTV-8 in Europe remains unclear. en
dc.description.librarian hb2013 en
dc.description.librarian ab2013
dc.description.sponsorship The Norwegian School of Veterinary Science (NVH), Tine Diary Cooperative (Norway), Animalia Meat and Poultry Research Centre (Norway) and Torstedt’s legacy (Norway). en
dc.description.uri locate/tvjl en
dc.identifier.citation Coetzee, P, Stokstad, M, Myrmel, M, Mutowembwa, P, Loken, T, Venter, EH & Van Vuuren, M 2013, 'Transplacental infection in goats experimentally infected with a European strain of bluetongue virus serotype 8', Veterinary Journal, vol. 197, no. 2, pp. 335-341. en
dc.identifier.issn 1090-0233 (print)
dc.identifier.other 10.1016/j.tvjl.2013.01.005
dc.identifier.other 7003904650
dc.identifier.other 7004572625
dc.identifier.other O-6953-2014
dc.identifier.other N-9080-2014
dc.language.iso en en
dc.publisher Elsevier en
dc.relation.requires Adobe Acrobat Reader en
dc.rights © 2013 Elsevier. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Veterinary Journal. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Veterinary Journal, vol. 197, no. 2, pp. 335-341, 2013. doi : 10.1016/j.tvjl.2013.01.005 en
dc.subject Transplacental infection en
dc.subject Europe en
dc.subject Overwintering en
dc.subject Bluetongue virus serotype 8 en
dc.subject.lcsh Goats -- Diseases en
dc.subject.lcsh Bluetongue virus en
dc.title Transplacental infection in goats experimentally infected with a European strain of bluetongue virus serotype 8 en
dc.type Postprint Article en

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