dc.contributor.author |
Phillips, Sonia M.
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dc.contributor.author |
Dubery, Ian A.
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dc.contributor.author |
Van Heerden, Henriette
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dc.date.accessioned |
2013-11-12T10:41:34Z |
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dc.date.available |
2013-11-12T10:41:34Z |
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dc.date.issued |
2013-10 |
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dc.description.abstract |
The identification and molecular characterisation of two lipin-like gene copies (GhLIPN) in cotton, Gossypium hirsutum, an allotetraploid derived from two progenitor diploid Gossypium species, is described. Sequence analyses of the GhLIPN copies, designated GhLIPN-1 and -2, revealed that they contain 11 exons, separated by ten introns. They each have a 2,643 bp open reading frame that encodes 880 aa proteins, and share a 97.7 and 95.5 % sequence similarity at the translated nucleotide and amino acid level, respectively. The GhLIPN genes have a distinct domain architecture consisting of an archetypical N-terminal lipin domain, followed by a haloacid dehalogenase (HAD) domain towards the C-terminus. A Southern blot did not distinguish between the two gene copies, which suggests that they may be homoeologs rather than paralogs. GhLIPN-2 is more similar to a homoeologous sequence from G. raimondii, representing the ancestral D-genome, compared to GhLIPN-1 that matches G. herbaceum and that represents the A-genome. Our data indicate that GhLIPN-1 and GhLIPN-2 are homoeologs that derive from the A- and the D-diploid genomes, respectively. The promoter sequences of GhLIPN-1 and -2 differ by 56 %, as a result of multiple indels. In silico analysis of the promoter regions revealed that both genes contain numerous putative defence-related and elicitor-responsive cis-elements that support a role for GhLIPN in defence responses. Relative quantification real-time PCR confirmed the up-regulation in response to a cell-wall-derived V. dahliae elicitor, which supported the association of GhLIPN with defence signalling. The results add a new dimension to the proposed roles of lipins in plants by suggesting that lipins may have a role in defence signalling. |
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dc.description.librarian |
hb2013 |
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dc.description.librarian |
ab2013 |
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dc.description.sponsorship |
The South African National Research Foundation and the University of Johannesburg. |
en |
dc.description.uri |
http://link.springer.com/journal/438 |
en |
dc.identifier.citation |
Phillips, SM, Dubery, IA & Van Heerden, H 2013, 'Molecular characterisation of two homoeologous elicitor-responsive lipin genes in cotton', Molecular Genetics and Genomics, vol. 288, no.10, pp. 519-533. |
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dc.identifier.issn |
1617-4615 (print) |
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dc.identifier.issn |
1617-4623 (onine) |
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dc.identifier.other |
10.1007/s00438-013-0770-8 |
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dc.identifier.uri |
http://hdl.handle.net/2263/32378 |
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dc.language.iso |
en |
en |
dc.publisher |
Springer |
en |
dc.relation.requires |
Adobe Acrobat Reader |
en |
dc.rights |
© Springer-Verlag Berlin Heidelberg 2013. The original publication is available at : http://link.springer.com/journal/438 |
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dc.subject |
Allotetraploid |
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dc.subject |
Gossypium hirsutum |
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dc.subject |
Haloacid dehalogenase |
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dc.subject |
Homoeologs |
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dc.subject |
Lipin |
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dc.subject |
Phosphatidate phosphatase |
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dc.subject |
Verticillium dahliae |
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dc.subject.lcsh |
Cotton |
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dc.subject.lcsh |
Plant gene expression |
en |
dc.subject.lcsh |
Polymerase chain reaction |
en |
dc.subject.lcsh |
Genes |
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dc.title |
Molecular characterisation of two homoeologous elicitor-responsive lipin genes in cotton |
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dc.type |
Postprint Article |
en |