Deciphering the cryptic genome : genome-wide analyses of the rice pathogen Fusarium fujikuroi reveal complex regulation of secondary metabolism and novel metabolites

Show simple item record Wiemann, Philipp Sieber, Christian M.K. Von Bargen, Katharina W. Studt, Lena Niehaus, Eva-Maria Espino, Jose J. Huß, Kathleen Michielse, Caroline B. Albermann, Sabine Wagner, Dominik Bergner, Sonja V. Connolly, Lanelle R. Fischer, Andreas Reuter, Gunter Kleigrewe, Karin Bald, Till Wingfield, Brenda D. Ophir, Ron Freeman, Stanley Hippler, Michael Smith, Kristina M. Brown, Daren W. Proctor, Robert H. Munsterkotter, Martin Freitag, Michael Humpf, Hans-Ulrich Guldener, Ulrich Tudzynski, Bettina 2013-10-08T06:40:01Z 2013-10-08T06:40:01Z 2013-06-27
dc.description.abstract The fungus Fusarium fujikuroi causes ‘‘bakanae’’ disease of rice due to its ability to produce gibberellins (GAs), but it is also known for producing harmful mycotoxins. However, the genetic capacity for the whole arsenal of natural compounds and their role in the fungus’ interaction with rice remained unknown. Here, we present a high-quality genome sequence of F. fujikuroi that was assembled into 12 scaffolds corresponding to the 12 chromosomes described for the fungus. We used the genome sequence along with ChIP-seq, transcriptome, proteome, and HPLC-FTMS-based metabolome analyses to identify the potential secondary metabolite biosynthetic gene clusters and to examine their regulation in response to nitrogen availability and plant signals. The results indicate that expression of most but not all gene clusters correlate with proteome and ChIP-seq data. Comparison of the F. fujikuroi genome to those of six other fusaria revealed that only a small number of gene clusters are conserved among these species, thus providing new insights into the divergence of secondary metabolism in the genus Fusarium. Noteworthy, GA biosynthetic genes are present in some related species, but GA biosynthesis is limited to F. fujikuroi, suggesting that this provides a selective advantage during infection of the preferred host plant rice. Among the genome sequences analyzed, one cluster that includes a polyketide synthase gene (PKS19) and another that includes a non-ribosomal peptide synthetase gene (NRPS31) are unique to F. fujikuroi. The metabolites derived from these clusters were identified by HPLC-FTMS-based analyses of engineered F. fujikuroi strains overexpressing cluster genes. In planta expression studies suggest a specific role for the PKS19-derived product during rice infection. Thus, our results indicate that combined comparative genomics and genome-wide experimental analyses identified novel genes and secondary metabolites that contribute to the evolutionary success of F. fujikuroi as a rice pathogen. en_US
dc.description.librarian am2013 en_US
dc.description.sponsorship The Deutsche Forschungsgesellschaft (DFG TU 101/16; HU 730/9; GU 1205/1, GU 1205/2) and by grants from the NIH (GM097637) and ACS (RSG-08-030-01-CCG) to MF. UG was funded by the Austrian Science Fund FWF (special research project Fusarium, F3705). en_US
dc.description.uri en_US
dc.identifier.citation Wiemann P, Sieber CMK, Von Bargen KW, Studt L, Niehaus E-M, et al. (2013) Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites. PLoS Pathog 9(6): e1003475. DOI: 10.1371/journal.ppat.1003475 en_US
dc.identifier.issn 1553-7366
dc.identifier.other 10.1371/journal.ppat.1003475
dc.language.iso en en_US
dc.publisher Public Library of Science en_US
dc.rights © 2013. The Authors. Licensee: PLoS. This work is licensed under the Creative Commons Attribution License. en_US
dc.subject Cryptic genome en_US
dc.subject Fusarium fujikuroi en_US
dc.subject Rice pathogen en_US
dc.title Deciphering the cryptic genome : genome-wide analyses of the rice pathogen Fusarium fujikuroi reveal complex regulation of secondary metabolism and novel metabolites en_US
dc.type Article en_US

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