In vivo effects of a novel calcium antagonist (R56865) against induced epoxyscillirosidin and tulp poisoning in sheep

Show simple item record Swan, Gerry E. Schultz, R.A. (Rowena Anitra) Kellerman, T. Stephanus Mulders, M.S.G. Maartens, B.P. Van der Walt, J.J.
dc.contributor.editor Verwoerd, Daniel Wynand 2013-09-12T08:20:29Z 2013-09-12T08:20:29Z 2013 1995
dc.description The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en
dc.description.abstract Two anaesthetized sheep were intoxicated with epoxyscillirosidin, the main cardio-active bufadienolide, extracted from Homeria pallida (Natal yellow tulp). The epoxyscillirosidin was injected intravenously as a bolus of 50 μg/kg, followed 30 min later by a continuous infusion in a normal saline drip (0,9% NaCI) at 25 μg/kg/h. In addition, another two conscious sheep were poisoned by intraruminal dosing of 1,25 g/kg of dried H. pallida plant material. Electrocardiograms, heart and respiratory rates and venous-acid-base levels were recorded prior to and at approximately 30-60 min intervals during the course of the experiment. Additional recordings were made when animals showed signs of intoxication. R56865 (Janssen Pharmaceutica, Pty Ltd), a novel Ca⁺⁺ antagonist, was administered at the first distinct signs of cardiac disturbances in the sheep given epoxyscillirosidin and after development of tachycardia and dyspnoea in those that received plant material. Activated charcoal was drenched at 3 g/kg to both sheep that received H. pallida about 1 h after the initial administration of R56865. All H. pallida sheep and one of the epoxyscillirosidin sheep survived. The signs of intoxication with H. pallida, namely groaning and tachypnoea, abated within minutes of treatment with R56865, but returned c. 30 min later in both animals. The treatment apparently had little effect on heart rate and EKG changes. One of the epoxyscillirosidin sheep was treated while exhibiting paroxysmal ventricular tachycardia. Although a transient improvement in conduction disturbance was recorded, the animal died soon afterwards. The results of this study indicate that the in vivo response of R56865 against induced bufadienolide cardiac disturbance in sheep is not as evident as that observed with R56865 against similar cardiac disturbances in vitro. The potential use of R56865 together with activated charcoal is discussed. en
dc.description.librarian mn2013
dc.identifier.citation Swan, GE, Schultz, RA, Kellerman, TS, Mulders, MSG, Maartens, BP & Van der Walt, JJ 1995, 'In vivo effects of a novel calcium antagonist (R56865) against induced epoxyscillirosidin and tulp poisoning in sheep’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 3, pp. 163-166. en
dc.identifier.issn 0330-2465
dc.identifier.other 7102127047
dc.identifier.other I-7222-2013 
dc.language.iso en en
dc.publisher Published by the Agricultural Research Council, Onderstepoort Veterinary Institute en
dc.rights © ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital). en
dc.subject Veterinary medicine en
dc.subject In vivo effects en
dc.subject Epoxyscillirosidin en
dc.subject Tulp poisoning en
dc.subject Sheep en
dc.subject Homeria pallida en
dc.subject Induced bufadienolide cardiac disturbance en
dc.subject Calcium antagonist (R56865) en
dc.subject.lcsh Veterinary medicine -- South Africa
dc.title In vivo effects of a novel calcium antagonist (R56865) against induced epoxyscillirosidin and tulp poisoning in sheep en
dc.type Article en

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