dc.contributor.advisor |
Van Heerden, Henriette |
|
dc.contributor.postgraduate |
Gomo, Calvin |
|
dc.date.accessioned |
2013-09-09T12:12:45Z |
|
dc.date.available |
2011-10-18 |
en |
dc.date.available |
2013-09-09T12:12:45Z |
|
dc.date.created |
2011-09-09 |
en |
dc.date.issued |
2011-10-18 |
en |
dc.date.submitted |
2011-10-18 |
en |
dc.description |
Dissertation (MSc)--University of Pretoria, 2011. |
en |
dc.description.abstract |
Brucellosis is an important zoonotic disease of ruminants, suidae, canids, several
wildlife species and humans caused by from the genus Brucella genus consisting of
gram-negative bacteria that are facultative intracellular pathogens. Brucellosis is
endemic in sub-Saharan African countries, which include Zimbabwe where Brucella
abortus and B. melitensis have been reported. In order to control brucellosis,
surveillance and identification of Brucella species is of paramount importance. The
aim of the study was to carry out a survey of bovine brucellosis using serological
tests, PCR assay and characterizing Brucella spp in Zimbabwe using molecular
techniques. Serological tests and PCR based assay were used to detect brucellosis
in cattle and wildlife in Chiredzi district of Zimbabwe. Blood and serum samples from
cattle (n=700) from Chiredzi district (Malipati and Pesvi communal areas), as well as,
African buffalo (n=10) and impalas (n=14) (from Gonarezhou National Park (GNP))
were tested for bovine brucellosis using rose bengal test (RBT), serum agglutination
methods (SAT) and indirect enzyme linked immunoassays (iELISA). The
seroprevalence of bovine brucellosis in cattle was found to be 8.3% using RBT and
iELISA and 6.0% using RBT, SAT and iELISA. No antibodies for Brucella spp were
detected in the African Buffaloes and impalas. Brucella strains were cultured from milk and blood sample from cows in Chiredzi district. DNA from the two Brucella
strains was amplified using the ITS66 and ITS279 primers specific for Brucella 16-
23S rDNA intergenic spacer (ITS) region. This Brucella specific PCR assay was
also used to detect Brucella DNA in blood and buffy coat samples from cattle that
were seropositive using RBT, SAT and iELISA. Despite the fact that the blood and
buffy coat samples were from animals that were bacteriemia since Brucella was
isolated from blood and milk and all samples had SAT titres above 148, no Brucella
DNA were detected using the specific PCR assay. Furthermore Brucella strains (23)
isolated at the Central Veterinary Laboratory (CVL) in Zimbabwe were identified
using molecular techniques like MLVA, AMOS-PCR and Bruceladder PCR. Only a
few isolates were classified up to species level using bacteriology method
(biotyping). MLVA-16 was able to identify B. abortus, B. melitensis, B. ovis and B.
suis strains amongst Zimbabwean isolates. The latter two species is the first report
of these species in Zimbabwe. The MLVA assay furthermore indicated that some
Zimbabwean isolates significantly differ from isolates of other origin in specific
species clusters. These Zimbabwean isolates could not be speciated and future
research using bacteriology and molecular characterization are necessary to
characterize these isolates. A few strains identified using bacteriological methods
and MLVA were also verified using AMOS-PCR and Bruceladder. For these results it
is clear that MLVA, AMOS-PCR and Bruceladder can be used to identify Brucella
species and biovars and can therefore contribute towards the control of brucellosis in
African countries. |
en |
dc.description.availability |
Unrestricted |
en |
dc.description.department |
Veterinary Tropical Diseases |
en |
dc.identifier.citation |
Gomo, C 2011, Characterisation of Brucella species from Zimbabwe, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-10182011-141615/ > |
en |
dc.identifier.other |
E11/9/267/hj |
|
dc.identifier.upetdurl |
http://upetd.up.ac.za/thesis/available/etd-10182011-141615/ |
en |
dc.identifier.uri |
http://hdl.handle.net/2263/31360 |
|
dc.language.iso |
|
en |
dc.publisher |
University of Pretoria |
|
dc.rights |
© 2011, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
en |
dc.subject |
UCTD |
en |
dc.title |
Characterisation of Brucella species in Zimbabwe |
en |
dc.type |
Dissertation |
en |