Influence of Sutherlandia frutescens extracts on cell numbers, morphology and gene expression in MCF-7 cells
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Date
Authors
Stander, Barend Andre
Marais, Sumari
Steynberg, Tenille Jolene
Theron, D.
Joubert, Fourie
Albrecht, C.
Joubert, Annie M.
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Sutherlandia frutescens is a well-known South African herbal remedy traditionally used for stomach problems, internal cancers, diabetes, various inflammatory conditions and recently to improve the overall health in cancer and HIV/AIDS patients. The influence of crude Sutherlandia frutescens extracts (prepared with 70% ethanol) was investigated on cell numbers, morphology, and gene expression profiles in a MCF-7 human breast adenocarcinoma cell line. Time-dependent (24, 34, 48 and 72 h) and dose-dependent (0.5–2.5 mg/ml) studies were conducted utilizing spectrophotometrical analysis with crystal violet as DNA stain. A statistically significant decrease to 50% of malignant cell numbers was observed after 24 h of exposure to 1.5 mg/ml Sutherlandia frutescens extract when compared to vehicle-treated controls. Morphological characteristics of apoptosis including cytoplasmic shrinking, membrane blebbing and apoptotic bodies were observed after 24 h of exposure. A preliminary global gene expression profile was obtained by means of microarray analysis and revealed valuable information about the molecular mechanisms and signal transduction associated with 70% ethanolic Sutherlandia frutescens extracts.
Description
Keywords
South African herb, Sutherlandia frutescens, Antitumor, Tumor cell line, Microarrays
Sustainable Development Goals
Citation
Stander, BA, Marais, S, Steynberg, TJ, Theron, D, Joubert, F, Albrecht, C & Joubert, AM 2007,‘Influence of Sutherlandia frutescens extracts on cell numbers, morphology and gene expression in MCF-7 cells’, Journal of Ethnopharmacology, vol. 112, no. 2, pp. 312-318.[http://www.sciencedirect.com/science/journal/03788741]