The purpose of this study is to explore the differences within specific temperament traits on emotional processing bias. Participants with extremes in temperament traits Harm Avoidance and Novelty Seeking were categorised and their performance on a computerised neuropsychological test battery was investigated. First year psychology students at a residential university in South Africa were invited to participate in the original study. Processing of the data yielded a realised sample of 431 participants who completed the Emotions battery, which comprised of four tasks. The results show that processing of affective valence varies according to individual differences within specific temperament traits. The findings suggest a negative emotion processing bias in the High HA group in comparison to the low HA group. Furthermore, the impulsive and extroverted High NS group show an increased ability to process emotional faces in comparison to the low NS group. The implications of these findings are discussed in the context of putative risk factors for psychopathological disorders.