Identification of volatile organic compounds from Eucalyptus detected by Gonipterus scutellatus (Gyllenhal) females

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dc.contributor.advisor Rohwer, Egmont Richard en
dc.contributor.coadvisor Slippers, Bernard en
dc.contributor.postgraduate Bouwer, Marc Clement
dc.date.accessioned 2013-09-07T15:36:43Z
dc.date.available 2010-11-11 en
dc.date.available 2013-09-07T15:36:43Z
dc.date.created 2010-09-02 en
dc.date.issued 2010-11-11 en
dc.date.submitted 2010-11-11 en
dc.description Dissertation (MSc)--University of Pretoria, 2010. en
dc.description.abstract This thesis concerns the development of semiochemical identification expertise and methodology at the University of Pretoria. The Eucalyptus snout beetle Gonipterus scutellatus was used as a model insect in developing these methods, firstly because it is a known pest in the Eucalyptus forestry industry of South Africa. Secondly, nothing is known about its chemical ecology and lastly, it is a relatively large insect that is easily worked on. Three main techniques were used namely: Electroantennography (EAG), Gas Chromatography Electroantennography Detection (GC-EAD) and Gas Chromatog- raphy Mass Spectrometry (GC-MS). EAG was used to difierentiate and identify certain Eucalyptus species that were expected to contain compounds that may function as either kairomones or allomones for G. scutellatus. The EAG process revealed that G. scutellatus responds more intensely to damaged Eucalyptus leaves as compared to undamaged leaves. The crushed foliage of the known hosts Eucalyptus globulus and E. viminalis gave larger responses than the crushed foliage from a known non-host E. citriodora. We sampled the volatiles from the crushed foliage of these three species and tentatively identified sixteen compounds from the E. globulus volatile profile that was antennally active for G. scutellatus females. The presence of these volatiles were subsequently investigated for E. viminalis and E. citriodora. The green leaf volatiles, (Z)-3 hexenyl acetate, (Z)-3-hexen-1-ol and (E)-2-hexenal and aromatic compounds, 2-phenylethanol, benzyl acetate and ethylphenylacetate often gave larger responses than the terpenes such as α-pinene, β-pinene and camphene. Crushed E. globulus leaves contained 2-phenyl ethanol, benzyl acetate, ethylphenylacetate, eucalyptol, α-pinene, (Z)-3 hexenyl acetate, (Z)-3-hexen-1-ol and (E)-2-hexenal that were antennally active. The E. viminalis profile had very little 2-phenylethanol and virtually no benzyl acetate. The E. citriodora volatile profile contained very little (Z)-3-hexen-1-ol, (E)-2-hexenal, 2-phenylethanol, benzyl acetate and ethylphenylacetate. These compounds may influence the host selection behaviour of G. scutellatusfemales. These volatiles can be tested in a behavioural bioassay in order to determine their effect on the Eucalyptus snout beetle G. scutellatus. en
dc.description.availability unrestricted en
dc.description.department Chemistry en
dc.identifier.citation Bouwer, MC 2010, Identification of volatile organic compounds from Eucalyptus detected by Gonipterus scutellatus (Gyllenhal) females, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/29414 > en
dc.identifier.other E10/739/ag en
dc.identifier.upetdurl http://upetd.up.ac.za/thesis/available/etd-11112010-152730/ en
dc.identifier.uri http://hdl.handle.net/2263/29414
dc.language.iso en
dc.publisher University of Pretoria en_ZA
dc.rights © 2010 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. en
dc.subject Snout beetle gonipterus scutellatus en
dc.subject Eucalyptus globulus en
dc.subject e. viminalis en
dc.subject Non-host e. citriodora en
dc.subject Volatile organic compounds en
dc.subject UCTD en_US
dc.title Identification of volatile organic compounds from Eucalyptus detected by Gonipterus scutellatus (Gyllenhal) females en
dc.type Dissertation en


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