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dc.contributor.author | Boy, Sonja Catharina![]() |
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dc.contributor.author | Janse van Rensburg, Estrelita![]() |
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dc.contributor.author | Engelbrecht, Susan![]() |
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dc.contributor.author | Dreyer, Leonora![]() |
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dc.contributor.author | Van Heerden, Marlene B.![]() |
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dc.contributor.author | Van Heerden, Willem Francois Petrus![]() |
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dc.date.accessioned | 2007-06-15T08:24:54Z | |
dc.date.available | 2007-06-15T08:24:54Z | |
dc.date.issued | 2006-02 | |
dc.description.abstract | Background: High-risk human papilloma viruses (HPV) are reported to be significant independent risk factors for oral squamous cell carcinoma (OSCC). The prevalence of HPV in OSCC in a South African population sample was evaluated comparing three different HPV detection methods. Methods: Tumour and adjacent morphologically normal oral mucosa of 59 resections of primary OSCC were evaluated for the presence of HPV using real-time polymerase chain reaction (PCR), conventional in situ hybridization (ISH), and a signal amplification ISH technique (Dako GenPointTM). Results: HPV18 DNA was detected in seven cases using real-time PCR. No positivity was found with the other two ISH techniques. Conclusions: We support the view that HPV is probably unimportant in the pathogenesis of OSCC and hypothesize HPV detection techniques as the main reason for the positive results in many studies. Real-time PCR was confirmed as the most sensitive technique, but researchers are urged to incorporate strict internal controls when using this detection method. | en |
dc.format.extent | 120713 bytes | |
dc.format.mimetype | application/pdf | |
dc.identifier.citation | Boy, S, Janse van Rensburg, E, Engelbrecht, S, Dreyer, L, Van Heerden, M & Van Heerden, W 2006, 'HPV detection in primary intra-oral squamous cell carcinomas – commensal, aetiological agent or contamination?', Journal of Oral Pathology and Medicine, vol. 35, no. 2, pp. 86-90. [http://www.blackwell-synergy.com/loi/jop] | en |
dc.identifier.issn | 0904-2512 | |
dc.identifier.other | 10.1111/j.1600-0714.2006.00385.x | |
dc.identifier.uri | http://hdl.handle.net/2263/2736 | |
dc.language.iso | en | en |
dc.publisher | Blackwell | en |
dc.rights | The definitive version is available at www.blackwell-synergy.com | en |
dc.subject | Human papillomavirus (HPV) | en |
dc.subject | Intra-oral squamous cell carcinoma | en |
dc.subject | Oral cancer | en |
dc.subject | Real-time PCR | en |
dc.subject | Oral neoplasms | en |
dc.subject.lcsh | Papillomaviruses | en |
dc.subject.lcsh | In situ hybridization | en |
dc.subject.lcsh | Squamous cell carcinoma (SCC) | en |
dc.subject.lcsh | Mouth -- Cancer | en |
dc.subject.lcsh | Polymerase chain reaction | en |
dc.title | HPV detection in primary intra-oral squamous cell carcinomas – commensal, aetiological agent or contamination? | en |
dc.type | Postprint Article | en |