HPV detection in primary intra-oral squamous cell carcinomas – commensal, aetiological agent or contamination?

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dc.contributor.author Boy, Sonja Catharina
dc.contributor.author Janse van Rensburg, Estrelita
dc.contributor.author Engelbrecht, Susan
dc.contributor.author Dreyer, Leonora
dc.contributor.author Van Heerden, Marlene B.
dc.contributor.author Van Heerden, W.F.P. (Willem Francois Petrus), 1958-
dc.date.accessioned 2007-06-15T08:24:54Z
dc.date.available 2007-06-15T08:24:54Z
dc.date.issued 2006-02
dc.description.abstract Background: High-risk human papilloma viruses (HPV) are reported to be significant independent risk factors for oral squamous cell carcinoma (OSCC). The prevalence of HPV in OSCC in a South African population sample was evaluated comparing three different HPV detection methods. Methods: Tumour and adjacent morphologically normal oral mucosa of 59 resections of primary OSCC were evaluated for the presence of HPV using real-time polymerase chain reaction (PCR), conventional in situ hybridization (ISH), and a signal amplification ISH technique (Dako GenPointTM). Results: HPV18 DNA was detected in seven cases using real-time PCR. No positivity was found with the other two ISH techniques. Conclusions: We support the view that HPV is probably unimportant in the pathogenesis of OSCC and hypothesize HPV detection techniques as the main reason for the positive results in many studies. Real-time PCR was confirmed as the most sensitive technique, but researchers are urged to incorporate strict internal controls when using this detection method. en
dc.format.extent 120713 bytes
dc.format.mimetype application/pdf
dc.identifier.citation Boy, S, Janse van Rensburg, E, Engelbrecht, S, Dreyer, L, Van Heerden, M & Van Heerden, W 2006, 'HPV detection in primary intra-oral squamous cell carcinomas – commensal, aetiological agent or contamination?', Journal of Oral Pathology and Medicine, vol. 35, no. 2, pp. 86-90. [http://www.blackwell-synergy.com/loi/jop] en
dc.identifier.issn 0904-2512
dc.identifier.other 10.1111/j.1600-0714.2006.00385.x
dc.identifier.uri http://hdl.handle.net/2263/2736
dc.language.iso en en
dc.publisher Blackwell en
dc.rights The definitive version is available at www.blackwell-synergy.com en
dc.subject Human papillomavirus (HPV) en
dc.subject Intra-oral squamous cell carcinoma en
dc.subject Oral cancer en
dc.subject Real-time PCR en
dc.subject Oral neoplasms en
dc.subject.lcsh Papillomaviruses en
dc.subject.lcsh In situ hybridization en
dc.subject.lcsh Squamous cell carcinoma (SCC) en
dc.subject.lcsh Mouth -- Cancer en
dc.subject.lcsh Polymerase chain reaction en
dc.title HPV detection in primary intra-oral squamous cell carcinomas – commensal, aetiological agent or contamination? en
dc.type Postprint Article en


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