Stimulation of prostaglandin E2 (PGE2) production by arachidonic acid, oestrogen and parathyroid hormone in MG-63 and MC3T3-E1 osteoblast-like cells

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dc.contributor.author Coetzee, Magdalena
dc.contributor.author Haag, Marianne
dc.contributor.author Claassen, Nico
dc.contributor.author Kruger, Marlena C.
dc.date.accessioned 2007-06-08T10:27:28Z
dc.date.available 2007-06-08T10:27:28Z
dc.date.issued 2005-12
dc.description.abstract Polyunsaturated fatty acids (PUFAs) as well as oestrogen (E2) and parathyroid hormone (PTH) affect bone cells. The aim of the study was to determine whether arachidonic acid (AA), E2, and PTH increase prostaglandin E2 (PGE2) synthesis in MG-63 and MC3T3-E1 osteoblastic cells and the level of mediation by COX-1 and COX-2. PGE2 levels were determined in the conditioned culture media of MG-63 and MC3T3-E1 osteoblasts after exposure to AA, PTH and E2. Cells were pre-incubated in some experiments with the unselective COX inhibitor indomethacin or the COX-2 specific blocker NS-398. Indirect immunofluorescence was performed on MG-63 cells to detect the presence and location of the two enzymes involved. AA increased PGE2 secretion in both cell lines; production by MC3T3-E1 cells, however, was significantly higher than that of MG-63 cells. This could be due to autoamplification via the EP1 subtype of PGE receptors in mouse MC3T3-E1 osteoblasts. Both COX-1 and COX-2 affected the regulation of PGE2 synthesis in MG-63 cells. E2 had no effect on PGE2 secretion in both cell lines, while PTH caused a slight increase in PGE2 synthesis in the MG-63 cell line. en
dc.description.sponsorship This material is based upon work supported by the National Research Foundation, South Africa under grant number 2053854 en
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dc.format.mimetype application/pdf
dc.identifier.citation Coetzee, M, Haag, M, Claassen, N & Kruger, MC 2005, ‘Stimulation of prostaglandin E2 (PGE2) production by arachidonic acid, oestrogen and parathyroid hormone in MG-63 and MC3T3-E1 osteoblast-like cells’, Prostaglandins, Leukotrienes and Essential Fatty Acids, vol. 73, no. 6, pp. 423-430. [http://www.sciencedirect.com/science/journal/09523278] en
dc.identifier.issn 0952-3278
dc.identifier.other 10.1016/j.plefa.2005.08.005
dc.identifier.uri http://hdl.handle.net/2263/2677
dc.language.iso en en
dc.publisher Elsevier en
dc.rights Elsevier en
dc.subject Polyunsaturated fatty acids en
dc.subject Oestrogen en
dc.subject.lcsh Unsaturated fatty acids
dc.subject.lcsh Estrogen
dc.subject.lcsh Parathyroid hormone (PTH)
dc.subject.lcsh Prostaglandins
dc.subject.lcsh Arachidonic acid
dc.title Stimulation of prostaglandin E2 (PGE2) production by arachidonic acid, oestrogen and parathyroid hormone in MG-63 and MC3T3-E1 osteoblast-like cells en
dc.type Postprint Article en


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