Clinical relevance of Salmonella enterica isolated from water and food in Eritrea

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dc.contributor.advisor Venter, S.N. (Stephanus Nicolaas) en
dc.contributor.postgraduate Said, H.M. (Halima Mohammed) en
dc.date.accessioned 2013-09-07T05:21:02Z
dc.date.available 2006-02-01 en
dc.date.available 2013-09-07T05:21:02Z
dc.date.created 2005-01-21 en
dc.date.issued 2007-02-01 en
dc.date.submitted 2006-02-01 en
dc.description Dissertation (MSc (Microbiology))--University of Pretoria, 2007. en
dc.description.abstract A total of 94 Salmonella isolates were collected from three catchments areas in Eritrea. These isolates were recovered from clinical and environmental sources. Biochemical tests using gelatin hydrolysis and tartrate utilization test were employed to differentiate between Salmonellasubspecies. All Salmonellaisolates were identified as Salmonellasubspecies I and were then subjected to molecular characterization. Polymerase chain reaction (PCR) and amplified fragment length polymorphism (AFLP) were employed to identify and establish possible relationships between the clinical isolates and environmental sources. Two sets of oligonucleotide primers specific for genes from S. Typhimurium and S. Enteritidis were used for the PCR reaction. Of the 94 Salmonellaisolates characterized only 6 were S. Typhimurium strains. To type the Salmonellaisolates AFLP was used. Clustering the AFLP patterns using the un-weighed pair-group method using arithmetic means (UPGMA) revealed 15 clusters. Of the 94 Salmonellaisolates collected, 48 (51%) strains were serologically identified. These serotypes include, 21 SalmonellaEmek (43.7%), 19 SalmonellaHeidelberg (39.5%), 7 of the 13, 22, 23; z undetermined serotype (14.5%), and 2 SalmonellaTyphimurium strains (4.1%). The AFLP data in the present study indicated a possible relationship between the clinical isolates and those obtained from environmental sources. en
dc.description.availability unrestricted en
dc.description.department Microbiology and Plant Pathology en
dc.identifier.citation Said, H 2005, Clinical relevance of Salmonella enterica isolated from water and food in Eritrea, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/26422 > en
dc.identifier.upetdurl http://upetd.up.ac.za/thesis/available/etd-02012006-112757/ en
dc.identifier.uri http://hdl.handle.net/2263/26422
dc.language.iso en
dc.publisher University of Pretoria en_ZA
dc.rights © 2005, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. en
dc.subject No key words available en
dc.subject UCTD en_US
dc.title Clinical relevance of Salmonella enterica isolated from water and food in Eritrea en
dc.type Dissertation en


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