The specific objectives of this study were to determine the Newcastle disease virus (NDV) antibody titres from the chicken sera collected from various districts and provinces of Zambia and to determine the seroprevalence of ND in Zambian backyard chickens. Results showed that 73.9 % of the birds sampled tested positive for Newcastle disease (ND) antibodies. The seroprevalence of Newcastle disease virus (NDV) in Zambian backyard chicken flocks varied among the five provinces sampled, ranging from 82.6 % in Eastern Province to 48.3 % in Luapula Province. The seroprevalence of the virus also varied among the 11 districts sampled, ranging from 91.3 % in Monze District of Southern Province to 22.8 % in Mufulira District of the Copperbelt Province. The results indicated that the seroprevalence of ND in Zambia has increased since the last survey conducted in 1994. The data generated is expected to contribute towards a more clear understanding of the epidemiology of NDV that would ultimately contribute towards an improved ND control programme to benefit all stakeholders in Zambia. An improved ND control programme is expected to enhance flock numbers and ultimately improve the dietary requirements and income needs of many poor households in the country.