Isolation of an anti-HIV compound from Elaeodendron croceum (Thunb.) DC.

Show simple item record

dc.contributor.advisor Meyer, J.J.M. (Jacobus Johannes Marion) en
dc.contributor.postgraduate Prinsloo, Gerhard en
dc.date.accessioned 2013-09-06T20:47:45Z
dc.date.available 2007-06-08 en
dc.date.available 2013-09-06T20:47:45Z
dc.date.created 2007-04-20 en
dc.date.issued 2007-06-08 en
dc.date.submitted 2007-06-08 en
dc.description Thesis (PhD (Plant Physiology))--University of Pretoria, 2007. en
dc.description.abstract HIV/AIDS threaten more than 40 million people worldwide and more than 5 million in South Africa alone. There is no cure for the disease yet, and novel drugs need to be discovered to make any progress in combating the disease. Twelve extracts from indigenous South African plants were analysed, of which one, Elaeodendron croceum, showed exceptionally good inhibition of transcription factors and a recombinant HIV strain in the HeLa-TAT-Luc and MT-2 VSV-pseudotyped recombinant virus assays. The pure compound isolated from this extract seemed to be the most toxic of all the samples, with toxicity of only 25% at a concentration of 100 _g/ml. When the concentration is increased, the toxicity increased slowly from 15% at a concentration of 0.195 _g/ml until it reached 25% toxicity at a concentration of 100 _g/ml. The active concentration of the compound against HIV is much lower at 100 ng/ml with an inhibition of approximately 90% of the recombinant virus. The therapeutic index of 250 makes it a promising possibility to be studied further for the compound to be used as a drug. The semi-purified extract and the pure compound were tested for its toxicity on VERO cells. The semi-purified extract had no toxicity up to a concentration of 50 _g/ml and the pure compound had toxicity of 20 % up to a concentration of 25_g/ml. The active concentration of 100 ng/ml for the VSV-Pseudotype assay is much lower than the start of toxicity at 25 _g/ml, and leaves a margin of activity before the toxicity level is reached. Both the extract and pure compound shows promising results in vitro to be developed into a medicine to be used against HIV, but need more research on the effects in vivo. Using an extract is easier, cheaper and faster than isolating a pure compound from the extract. It might also be possible that the extract could be prepared as a tea and its use could be very accessible. en
dc.description.availability unrestricted en
dc.description.department Plant Science en
dc.identifier.citation Prinsloo, G 2007, Isolation of an anti-HIV compound from Elaeodendron croceum (Thunb.) DC., PhD thesis, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/25336 > en
dc.identifier.upetdurl http://upetd.up.ac.za/thesis/available/etd-06082007-164348/ en
dc.identifier.uri http://hdl.handle.net/2263/25336
dc.language.iso en
dc.publisher University of Pretoria en_ZA
dc.rights © 2007, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. en
dc.subject An anti-hiv compound en
dc.subject Elaeodendron croceum (thunb.) dc en
dc.subject Isolation en
dc.subject UCTD en_US
dc.title Isolation of an anti-HIV compound from Elaeodendron croceum (Thunb.) DC. en
dc.type Thesis en


Files in this item

This item appears in the following Collection(s)

Show simple item record