Molecular characterization of tick-borne pathogens of domestic dogs from communal areas in Botswana

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dc.contributor.advisor Penzhorn, Barend Louis en
dc.contributor.advisor Matjila, P.T. (Paul Tshepo) en
dc.contributor.postgraduate Sibanda, Donald Ray en
dc.date.accessioned 2013-09-06T18:55:21Z
dc.date.available 2012-06-01 en
dc.date.available 2013-09-06T18:55:21Z
dc.date.created 2012-04-13 en
dc.date.issued 2011 en
dc.date.submitted 2012-05-24 en
dc.description Dissertation (MSc)--University of Pretoria, 2011. en
dc.description.abstract The occurrence of tick-borne pathogens in dogs on communal land in Botswana was studied using Reverse Line Blot (RLB). Eighty blood samples were collected from dogs that underwent a spay and neuter program in Maun, Botswana. These were sent to the Department of Veterinary Tropical Diseases, University of Pretoria, and were stored at the Molecular Laboratory. DNA was extracted from the samples followed by the Polymerase Chain Reaction (PCR) and the RLB. Six samples were found to be positive for Ehrlichia canis. Two of the samples were positive for a concurrent infection of E. canis and Ehrlichia ruminantium. There was one sample that was positive for the non-pathogenic Ehrlichia sp. Omatjenne. Although six samples were positive for the Babesia/Theileria catch-all probe, none hybridised with any of the species-specific probes. Copyright en
dc.description.availability unrestricted en
dc.description.department Veterinary Tropical Diseases en
dc.identifier.citation Sibanda, D R 2011, Molecular characterization of tick-borne pathogens of domestic dogs from communal areas in Botswana, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/24948 > en
dc.identifier.other E12/4/306/gm en
dc.identifier.upetdurl http://upetd.up.ac.za/thesis/available/etd-05242012-144516/ en
dc.identifier.uri http://hdl.handle.net/2263/24948
dc.language.iso en
dc.publisher University of Pretoria en_ZA
dc.rights © 2011, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria en
dc.subject Domestic dogs en
dc.subject Botswana en
dc.subject Tick-borne pathogens en
dc.subject UCTD en_US
dc.title Molecular characterization of tick-borne pathogens of domestic dogs from communal areas in Botswana en
dc.type Dissertation en


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