Genetic, antigenetic and phenotypic comparison of herpesviruses isolated from domestic and wild fields

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dc.contributor.advisor Venter, Estelle Hildegard en
dc.contributor.advisor Van Vuuren, Moritz en
dc.contributor.postgraduate Kashwantale, Eulalie en
dc.date.accessioned 2013-09-06T14:02:23Z
dc.date.available 2010-03-12 en
dc.date.available 2013-09-06T14:02:23Z
dc.date.created 2009-11-27 en
dc.date.issued 2010-03-12 en
dc.date.submitted 2010-03-02 en
dc.description Dissertation (MSc (Veterinary Tropical Diseases))--University of Pretoria, 2010. en
dc.description.abstract Feline herpesviruses are endemic in free-ranging lions in South Africa. Serological surveillance among free-living felids revealed high levels of exposure to the virus. However, clinical disease in wild felids following FHV-1 infection has been only described in captive populations and reported to be similar to that in the domestic cat. To expand the epidemiological understanding of feline herpesviruses in felids and for disease control, three strains of FHV-1 isolated from a domestic cat (Felis catus) a cheetah (Acinonyx jubatus) and an African wild cat (Felis silvestris) have been compared to determine their relatedness. A region of the herpesvirus DNA polymerase gene was amplified in a nested PCR with consensus degenerate primers to confirm the identity of the isolates. The genetic relatedness were investigated by comparing patterns of genomic DNA cleaved with restriction enzymes SalI and KpnI and the DNA fingerprints generated by different RAPD primers. For antigenic relationships, a panel of nine monoclonal antibodies prepared against a vaccine strain used against domestic cats were tested in a microneutralization assay. In addition, the phenotypic characteristics of the isolates were also compared by their ability to produce plaques in CrFK monolayer cell cultures. With restriction enzyme analysis, it was not possible to make a comparison due to lack of digestion of the genomic DNA of the domestic cat isolate. However, the RAPD-PCR revealed that isolates were closely related but distinct from each other. Only two monoclonal antibodies reacted with the wild isolates; an effect similar to a toxic effect on cell was observed with the domestic isolate. No significant differences of plaque production were observed among the trains. This study provides evidence of a closer evolutionary relationship between the three isolates. The results of the relationships based on the genetic and phenotypic characterization agreed well and both indicated that the viruses from the domestic and wild felids are different but have a high degree of similarity. Copyright en
dc.description.availability unrestricted en
dc.description.department Veterinary Tropical Diseases en
dc.identifier.citation Kashwantale, E 2009, Genetic, antigenetic and phenotypic comparison of herpesviruses isolated from domestic and wild fields, MSc (Veterinary Tropical Diseases) dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/22934 > en
dc.identifier.other E10/34/gm en
dc.identifier.upetdurl http://upetd.up.ac.za/thesis/available/etd-03022010-122006/ en
dc.identifier.uri http://hdl.handle.net/2263/22934
dc.language.iso en
dc.publisher University of Pretoria en_ZA
dc.rights © 2009, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. en
dc.subject Viruses en
dc.subject Domestic cats en
dc.subject Herpesviruses en
dc.subject South Africa en
dc.subject Lions en
dc.subject UCTD en_US
dc.title Genetic, antigenetic and phenotypic comparison of herpesviruses isolated from domestic and wild fields en
dc.type Dissertation en


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