Bluetongue and epizootic haemorrhagic disease virus in local breeds of cattle in Kenya

Show simple item record Toye, Philip G. Batten, C.A. Kiara, Henry Henstock, M.R. Edwards, L. Thumbi, Samuel Mwangi Poole, Elizabeth Jane Handel, Ian Graham Bronsvoort, Barend Mark de Clare Hanotte, Olivier Coetzer, Jacobus A.W. Woolhouse, Mark E.J. Oura, C.A.L. 2013-07-12T07:52:54Z 2013-07-12T07:52:54Z 2013-03
dc.description.abstract The presence of bluetongue virus (BTV) and Epizootic Haemorrhagic Disease virus (EHDV) in indigenous calves in western Kenya was investigated. Serum was analysed for BTV and EHDV antibodies. The population seroprevalences for BTV and EHDV for calves at 51 weeks of age were estimated to be 0.942 (95% CI 0.902–0.970) and 0.637 (95% CI 0.562–0.710), respectively, indicating high levels of circulating BTV and EHDV. The odds ratio of being positive for BTV if EHDV positive was estimated to be 2.57 (95% CI 1.37– 4.76). When 99 calves were tested for BTV and EHDV RNA by real-time RT-PCR, 88.9% and 63.6% were positive, respectively. Comparison of the serology and real-time RT-PCR results revealed an unexpectedly large number of calves that were negative by serology but positive by real-time RT-PCR for EHDV. Eight samples positive for BTV RNA were serotyped using 24 serotype-specific real-time RT-PCR assays. Nine BTV serotypes were detected, indicating that the cattle were infected with a heterogeneous population of BTVs. The results show that BTV and EHDV are highly prevalent, with cattle being infected from an early age. en
dc.description.librarian hb2013 en
dc.description.librarian ab2013
dc.description.sponsorship The Wellcome Trust (Project 079445) en
dc.description.uri en
dc.identifier.citation Toye, PG ... et al 2013, 'Bluetongue and Epizootic Haemorrhagic Disease virus in local breeds of cattle in Kenya', Research in Veterinary Science, vol. 94, no. 3, pp. 769-773. en
dc.identifier.issn 0034-5288 (print)
dc.identifier.issn 1532-2661 (online)
dc.identifier.other 10.1016/j.rvsc.2012.11.001
dc.language.iso en en
dc.publisher Elsevier en
dc.relation.requires Adobe Acrobat Reader en
dc.rights © 2012 Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Research in Veterinary Science.Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Research in Veterinary Science, vol.94, no. 3, 2013, doi.: 10.1016/j.rvsc.2012.11.001. en
dc.subject Kenya en
dc.subject Prevalence en
dc.subject Bluetongue virus en
dc.subject Epizootic haemorrhagic disease virus en
dc.subject EHDV en_ZA
dc.subject.lcsh Cattle -- Diseases en
dc.subject.lcsh Communicable diseases in animals -- Kenya en
dc.subject.lcsh Bluetongue -- Kenya en
dc.subject.lcsh Bluetongue virus -- Kenya en
dc.title Bluetongue and epizootic haemorrhagic disease virus in local breeds of cattle in Kenya en
dc.type Postprint Article en

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