Application of a competitive internal amplification control for the detection of sapoviruses in wastewater

Loading...
Thumbnail Image

Authors

Murray, Tanya Y.
Mans, Janet
Van Zyl, Walda B.
Taylor, Maureen B.

Journal Title

Journal ISSN

Volume Title

Publisher

Springer

Abstract

In this study, a competitive internal amplification control (IAC) was constructed for application in the real-time reverse transcription-polymerase chain reaction detection of sapoviruses (SaVs). A SaV RNA standard was also created for quantification of the virus. The IAC was included in the screening of environmental samples for SaVs. From August 2010 to December 2011, 51 wastewater samples were collected from five provinces in South Africa. SaVs were found in 72.5 % (37/51) of samples, including four samples where detection was initially inhibited. SaV concentrations ranged from 4.24 × 103 to 1.31 × 106 copies/ml. The IAC successfully identified samples which contained inhibitors and inclusion of an IAC is necessary to ensure the prevalence of SaVs is accurately determined. SaVs are present at high concentrations in wastewater in several provinces of South Africa. This widespread occurrence indicates that SaV circulation in the South African population may be underestimated.

Description

Keywords

Calicivirus, Enteric virus, Sapovirus, Sewage, South Africa

Sustainable Development Goals

Citation

Murray, TY, Mans, J, Van Zyl, WB & Taylor, MB 2013, 'Application of a competitive internal amplification control for the detection of sapoviruses in wastewater', Food and Environmental Virology, vol. 5, no.1, pp. 61-68.