Morphological identification of parasitic nematode infective larvae of small ruminants and cattle : a practical lab guide

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dc.contributor.author Van Wyk, J.A. (Jan Aucamp)
dc.contributor.author Mayhew, Estelle
dc.date.accessioned 2013-05-27T06:53:40Z
dc.date.available 2013-05-27T06:53:40Z
dc.date.issued 2013-03-13
dc.description Scan this QR code with your smart phone or mobile device to read online. en
dc.description J.A.v.W. (University of Pretoria) was responsible for developing the novel approach to morphological identification of infective larvae of the common gastrointestinal nematode genera of small ruminants and cattle and wrote the manuscript. E.M. (University of Pretoria) was responsible for all the art work and finishing of the article. en
dc.description.abstract In 2004, a new concept was introduced for simplifying identification of larvae of the common nematodes of cattle, sheep and goats that comprises estimates of the lengths of the sheath tail extensions of infective third-stage larvae (L3) of each genus and/or species to that of Trichostrongylus spp., instead of having to be dependent only on measurements in micrometre. For example, if the mean length of the sheath tail extension (the extension of the sheath caudad, beyond the caudal tip of the larva) of Trichostrongylus colubriformis and Trichostrongylus axei is assumed to be ‘X’, then that of Haemonchus contortus is 2.0–2.7 ‘X’ – a difference that is not difficult to estimate. An additional new approach suggested now, particularly for L3 of species and/or genera difficult to differentiate (such as Chabertia ovina and Oesophagostomum columbianum), is to estimate the proportion of the larval sheath tail extension comprising a terminal thin, whip-like filament. For the experienced person, it is seldom necessary to measure more than one or two sheath tail extensions of L3 in a mixed culture, because the identity of most of the remaining L3 can thereafter be estimated in relation to those measured, without having to take further measurements. The aim of this article was to present the novel approach in the form of a working guide for routine use in the laboratory. To facilitate identification, figures and a separate organogram for each of small ruminants and cattle have been added to illustrate the distinguishing features of the common L3. en
dc.description.librarian am2013 en
dc.description.librarian ab2013
dc.description.sponsorship This work was supported by the EU ‘PARASOL’ (EU-FP6) and ‘RISC-NET’ projects, the latter under the ‘CIDLID’ call for applications, funded by a partnership between the Biotechnology and Biological Sciences Research Council, the Department for International Development and the Scottish Government. en
dc.description.uri http://www.ojvr.org en
dc.identifier.citation Van Wyk, J.A. & Mayhew, E., 2013, ‘Morphological identification of parasitic nematode infective larvae of small ruminants and cattle: A practical lab guide’, Onderstepoort Journal of Veterinary Research 80(1), Art. #539, 14 pages. http://dx.DOI.org/ 10.4102/ojvr.v80i1.539 en
dc.identifier.issn 0030-2465 (print)
dc.identifier.issn 2219-0635 (online)
dc.identifier.other 10.4102/ojvr.v80i1.539
dc.identifier.uri http://hdl.handle.net/2263/21545
dc.language.iso en en
dc.publisher AOSIS Open Journals en
dc.relation.requires Adobe Acrobat Reader en
dc.rights © 2013. The Authors. Licensee: AOSIS OpenJournals. This work is licensed under the Creative Commons Attribution License. en
dc.subject Cattle en
dc.subject Parasitic nematode infective larvae en
dc.subject Small ruminants en
dc.subject.lcsh Nematodes en
dc.subject.lcsh Cattle -- Parasites en
dc.subject.lcsh Sheep -- Parasites en
dc.subject.lcsh Ruminants -- Parasites en
dc.title Morphological identification of parasitic nematode infective larvae of small ruminants and cattle : a practical lab guide en
dc.type Article en


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