Genetic characterization of bovine viral diarrhoea (BVD) viruses : confirmation of the presence of BVD genotype 2 in Africa

Show simple item record Ularamu, H.G. (Hussaini Gulak) Sibeko-Matjila, Kgomotso Penelope Bosman, A.B. Venter, Estelle Hildegard Van Vuuren, Moritz 2013-05-27T06:45:25Z 2013-05-27T06:45:25Z 2013-01
dc.description.abstract Bovine viral diarrhoea virus (BVDV) has emerged as one of the economically important pathogens in cattle populations with a worldwide distribution causing a complex of disease syndromes. Two genotypes BVDV 1 and 2 exist and are discriminated on the basis of the sequence of the 5′ non-coding region (5 NCR) using real-time PCR. The use of the real-time PCR is more sensitive, specific, and less time consuming than a conventional PCR, and has reduced the risk of cross-contamination of samples. Limited information exists on BVDV genetic subtypes in South Africa. The aim of this study was to determine the genotypes of BVDV currently circulating in South African feedlots. A total of 279 specimens (219 tissue samples, 59 trans-tracheal aspirates and 1 blood sample) were collected from dead and living cattle with lesions or clinical signs compatible with BVDV infection. Pooled homogenates from the same animals were prepared and total RNA extracted. A screening test was performed on the pooled samples and positive pools were investigated individually. The Cador BVDV Type 1/2 RT-PCR Kit (Qiagen, Hilden, Germany) was used for the real-time PCR assay on a LightCycler® V2.0 real-time PCR machine (Roche Diagnostics, Mannheim, Germany). The results were read at 530 and 640 nm for BVDV 1 and 2 respectively. Bovine viral diarrhoea virus was detected in a total of 103 samples that included 91 tissue samples, 1 blood sample and 11 trans-tracheal aspirates. Eighty five (82.5%) of the strains were genotype 1 and 18 (17.5%) were genotype 2. Comparing the sequencing data, genotype 1 and 2 from the field strains did not cluster with vaccine strains currently used in feedlots in South Africa. The present study revealed the presence of BVDV genotype 2 in cattle in South Africa based on the high sequence similarity between genotype 2 field strains and strain 890 from North America. The presence of genotype 2 viruses that phylogenetically belong to different clusters and coexist in feedlots is consistent with the possibility of multiple virus introductions. These results represent the first documented evidence for the presence of BVDV genotype 2 in African cattle. en
dc.description.librarian hb2013 en
dc.description.librarian ab2013
dc.description.sponsorship Funding for the research was provided by Pfizer® South Africa, Vision pharmaceuticals, South Africa, Design Biologix, South Africa.. en
dc.description.uri en
dc.identifier.citation Ularamu, HG, Sibeko, KP, Bosman, AB, Venter, EH & Van Vuuren, M 2013, ' Genetic characterization of bovine viral diarrhoea (BVD) viruses : confirmation of the presence of BVD genotype 2 in Africa', Archives of Virology, vol. 158, no. 1, pp. 155-163. en
dc.identifier.issn 0304-8608 (print)
dc.identifier.issn 1432-8798 (online)
dc.identifier.other 10.1007/s00705-012-1478-5
dc.identifier.other 7003904650
dc.identifier.other 7004572625
dc.identifier.other 24332907600
dc.identifier.other O-6028-2014
dc.identifier.other N-9080-2014
dc.language.iso en en
dc.publisher Springer en
dc.relation.requires Adobe Acrobat Reader en
dc.rights © Springer-Verlag 2012. The original publication is available at en
dc.subject Genotypes en
dc.subject Cador BVDV Type 1 en
dc.subject Real-time polymerase chain reaction en
dc.subject Genetic characterization en
dc.subject 5′ non-coding region en
dc.subject Cador BVDV Type 2
dc.subject.lcsh Cattle -- Diseases en
dc.subject.lcsh Diarrhea in cattle en
dc.subject.lcsh Bovine viral diarrhoea virus (BVDV) en
dc.subject.lcsh Bovine viral diarrhoea en
dc.subject.lcsh Cattle -- Viruses en
dc.title Genetic characterization of bovine viral diarrhoea (BVD) viruses : confirmation of the presence of BVD genotype 2 in Africa en
dc.type Postprint Article en

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