dc.contributor.author |
Van Elst, Daan
|
|
dc.contributor.author |
Van Wyk, Braam
|
|
dc.contributor.author |
Schultz, Anitra
|
|
dc.contributor.author |
Prinsen, Els
|
|
dc.date.accessioned |
2013-05-06T07:00:02Z |
|
dc.date.available |
2013-05-06T07:00:02Z |
|
dc.date.issued |
2013-01 |
|
dc.description.abstract |
Plant poisoning of livestock is responsible for considerable economic losses in southern Africa.
Six plant species of the Rubiaceae family are known to cause gousiekte, a cardiac syndrome of
ruminants induced by ingestion of the toxic compound pavettamine. Progress in understanding
the aetiology of this disease is largely hampered by the variable toxicity of the plants and the absence of a quantification method for pavettamine. The pavettamine concentration in leaf
samples of Fadogia homblei, a known gousiekte causing plant, was analyzed by massspectrometry.
In the most apical leaf pair, the highest concentration of pavettamine was
detected. Distal leaves contained progressively less pavettamine. Besides a significant amount
of free pavettamine, most pavettamine was found to occur in a conjugated form. To which
molecules the pavettamine is conjugated remains unknown as is the function of conjugated
pavettamine in the development of gousiekte. All know gousiekte-causing plants contain
symbiotic bacteria in their leaves; it was hypothesized that these bacteria might be involved in
the production of pavettamine. However, analysis of in vitro cultures of the Fadogia homblei
endosymbiont revealed no production of pavettamine. Pavettamine is therefore not produced
by the bacteria alone. It is either the product of the interaction with the plant or solely produced
by the host. |
en_US |
dc.description.librarian |
hb2013 |
en_US |
dc.description.sponsorship |
This research was supported financially by the Fund for Scientific Research,
Flanders (F.W.O., G.0343.09N). D.V.E. holds a Ph.D. research grant from the Fund for
Scientific Research, Flanders (F.W.O., no. 1.1.722.10.N.00). |
en_US |
dc.description.uri |
http://www.journals.elsevier.com/phytochemistry/ |
en_US |
dc.identifier.citation |
Van Elst, D, Van Wyk, B, Schultz, A & Prinsen, E 2013, 'Production of toxic pavettamine and pavettamine conjugates in the gousiekte-causing Fadogia homblei plant and its relation to the bacterial endosymbiont', Phytochemistry, vol. 85, no. 1, pp. 92-98. |
en_US |
dc.identifier.issn |
0031-9422 (print) |
|
dc.identifier.issn |
1873-3700 (online) |
|
dc.identifier.other |
10.1016/j.phytochem.2012.08.025 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/21443 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Elsevier |
en_US |
dc.rights |
© 2013 Elsevier. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Phytochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Phytochemistry, vol. 85, no. 1, 2013, DOI :
10.1016/j.phytochem.2012.08.025 |
en_US |
dc.subject |
Fadogia homblei |
en_US |
dc.subject |
Rubiaceae |
en_US |
dc.subject |
Burkholderia |
en_US |
dc.subject |
Endosymbiont |
en_US |
dc.subject |
Mass-spectrometry |
en_US |
dc.subject |
Pavettamine |
en_US |
dc.subject |
Gousiekte |
en_US |
dc.title |
Production of toxic pavettamine and pavettamine conjugates in the gousiekte-causing Fadogia homblei plant and its relation to the bacterial endosymbiont |
en_US |
dc.type |
Postprint Article |
en_US |