Use of transgenic Oryzacystatin-I expressing plants enhances recombinant protein production

Show simple item record Pillay, Priyen Kibido, Tsholofelo Reineth Du Plessis, M. Van der Vyver, Christell Beyene, Getu Vorster, Barend Juan Kunert, Karl J. Schluter, Urte 2013-04-26T07:23:21Z 2013-04-26T07:23:21Z 2012-09-11
dc.description.abstract Plants are an effective and inexpensive host for the production of commercially interesting heterologous recombinant proteins. The Escherichia coli-derived glutathione reductase was transiently expressed as a recombinant model protein in the cytosol of tobacco plants using the technique of leaf agro-infiltration. Proteolytic cysteine protease activity progressively increased over time when glutathione reductase accumulated in leaves. Application of cysteine protease promoter–GUS fusions in transgenic tobacco identified a cysteine protease NtCP2 expressed in mature leaves and being stress responsive to be expressed as a consequence of agro-infiltration. Transgenic tobacco plants constitutively expressing the rice cysteine protease inhibitor oryzacystatin-I had significantly lower cysteine protease activity when compared to non-transgenic tobacco plants. Lower cysteine protease activity in transgenic plants was directly related to higher glutathione reductase activity and also higher glutathione reductase amounts in transgenic plants. Overall, our work has demonstrated as a novel aspect that transgenic tobacco plants constitutively expressing an exogenous cysteine protease inhibitor have the potential for producing more recombinant protein which is very likely due to the reduced activity of endogenous cysteine protease. en_US
dc.description.librarian hb2013 en_US
dc.description.sponsorship This work was supported by a grant from the National Research Foundation in South Africa. en_US
dc.description.uri en_US
dc.identifier.citation Pillay, P, Kibido, T, Du Plessis, M, Van der Vyver, C, Beyene, G, Vorster, BJ, Kunert, KJ & Schluter, U 2012, 'Use of transgenic Oryzacystatin-I expressing plants enhances recombinant protein production', Applied Biochemistry and Biotechnology, vol. 168, no. 6, pp. 1608-1620. en_US
dc.identifier.issn 0273-2289 (print)
dc.identifier.issn 1559-0291 (online)
dc.identifier.other 10.1007/s12010-012-9882-6
dc.language.iso en en_US
dc.publisher Springer en_US
dc.rights © Springer-Verlag 2012. The original publication is available at en_US
dc.subject Cysteine protease en_US
dc.subject Cysteine protease inhibitor en_US
dc.subject Transient expression en_US
dc.subject Tobacco agro-infiltration en_US
dc.subject Glutathione reductase en_US
dc.title Use of transgenic Oryzacystatin-I expressing plants enhances recombinant protein production en_US
dc.type Postprint Article en_US

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