The Tankwa Karoo National Park has been enlarged from 27 064 ha to 143 600 ha. This whole area is severely under-collected for plants in general and therefore it was an obvious target for the South African National Parks (SANParks) Programme, a component of the Pretoria National Herbarium (PRE) Plant Collecting Programme. This programme not only aims to survey national parks that have been poorly surveyed, but also inadequately known taxa, unique habitats, remote and inaccessible areas and plant species flowering at irregular times, especially after events such as fire or unusual timing of, or high, rainfall. General collecting in the Tankwa Karoo National Park has already led to the description of two new taxa, from two families. It furthermore resulted in new distribution records for the park and for the Northern Cape Province. These are reported on here. Conservation implications: Although the Tankwa Karoo National Park falls within the Succulent Karoo Biome (a biodiversity hotspot of international importance), information on its plant diversity is insufficient because it is an under-collected area. Results of this study will guide conservation and supply occurrence and distribution data required to compile management plans for the park.