Abstract:
Successful long term bone replacement and repair remain a challenge today. Nanotechnology
has makes it possible to alter materials’ characteristics and therefore possibly improve on the
material itself. In this study, biphasic (hydroxyapatite/β-tricalcium phosphate (HA/β-TCP))
nanobioceramic scaffolds were prepared by the electrospinning technique in order to mimic
the extracellular matrix (ECM). Scaffolds were characterised by scanning electron
microscopy (SEM) and Attentuated Total Reflectance Fourier Transform Infrared (ATRFTIR).
Osteoblasts as well as monocytes that were differentiated into osteoclast-like cells,
were cultured separately on the biphasic bioceramic scaffolds for up to 6 days and the
proliferation, adhesion and cellular response were determined using lactate dehydrogenase
(LDH) cytotoxicity assay, nucleus and cytoskeleton dynamics, analysis of the cell cycle
progression, measurement of the mitochondrial membrane potential and the detection of
phosphatidylserine expression. SEM analysis of the biphasic bioceramic scaffolds revealed
nano fibers spun in a mesh-like scaffold. Results indicate that the biphasic bioceramic
electrospun scaffolds are biocompatible and have no significant negative effects on either
osteoblasts or osteoclast-like cells in vitro.
