Differential signaling involved in Sutherlandia frutescens-induced cell death in MCF-7 and MCF-12A cells

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dc.contributor.author Vorster, C.J.J. (Christiaan Jacob Johan)
dc.contributor.author Stander, Andre
dc.contributor.author Joubert, Annie M.
dc.date.accessioned 2013-04-19T08:53:37Z
dc.date.available 2013-04-19T08:53:37Z
dc.date.issued 2012-03
dc.description.abstract Ethnopharmacological relevance: The scientific study of natural products traditionally used in anticancer preparations has yielded several therapeutically relevant compounds. One of these traditional preparations with potentially beneficial properties is aqueous extracts of Sutherlandia frutescens, a shrub indigenous to the Western Cape region of South Africa. Aims of the study: To evaluate in vitro efficacy of these preparations on the MCF-7 breast adenocarcinoma and MCF-12A non-tumorigenic cell lines in terms of cell proliferation, cell morphology and possible induction of cell death. Materials and Methods: Crystal violet staining was used to evaluate cell proliferation, lightand fluorescence microscopy were used to investigate both intracellular and extracellular morphological features of apoptosis and autophagy (e.g. membrane blebbing, condensed chromatin and intracellular lysosomes), while flow cytometry quantified cell cycle changes and induction of apoptosis through analysis of the flip-flop translocation of phosphatidylserine. en_US
dc.description.librarian hb2013 en_US
dc.description.sponsorship The Medical Research Council of South Africa (AK076; AL343). The Cancer Association of South Africa (AK246).The National Research Foundation of South Africa (AL239) and the Struwig-Germeshuysen Cancer Research Trust of South Africa (AN074). en_US
dc.description.uri http://www.elsevier.com/locate/jethpharm en_US
dc.identifier.citation Vorster, C, Stander, A & Joubert, A 2012, 'Differential signaling involved in Sutherlandia frutescens-induced cell death in MCF-7 and MCF-12A cells', Journal of Ethnopharmacology, vol. 140, no. 1, pp.123-130. en_US
dc.identifier.issn 0378-8741 (print)
dc.identifier.issn 1872-7573 (online)
dc.identifier.other 10.1016/j.jep.2011.12.045
dc.identifier.uri http://hdl.handle.net/2263/21329
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.rights © 2012 Elsevier Ireland Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Journal of Ethnopharmacology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Ethnopharmacology, vol. 140, issue 1, 2012, DOI. : 10.1016/j.jep.2011.12.045 en_US
dc.subject Sutherlandia frutescens en_US
dc.subject Cytotoxicity en_US
dc.subject Cancer en_US
dc.subject Ethnopharmacology en_US
dc.subject Apoptosis en_US
dc.subject Autophagy en_US
dc.title Differential signaling involved in Sutherlandia frutescens-induced cell death in MCF-7 and MCF-12A cells en_US
dc.type Postprint Article en_US


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