Sequence variation identified in the 18S rRNA gene of Theileria mutans and Theileria velifera from the African buffalo (Syncerus caffer)

Show simple item record Chaisi, Mamohale E. Collins, Nicola E. Potgieter, Fred T. Oosthuizen, Marinda C. 2013-04-17T06:42:33Z 2013-04-17T06:42:33Z 2013-01
dc.description.abstract The African buffalo (Syncerus caffer) is a natural reservoir host for both pathogenic and nonpathogenic Theileria species. These often occur naturally as mixed infections in buffalo. Although the benign and mildly pathogenic forms do not have any significant economic importance, their presence could complicate the interpretation of diagnostic test results aimed at the specific diagnosis of the pathogenic T. parva in cattle and buffalo in South Africa. The 18S rRNA gene has been used as the target in a quantitative real-time PCR (qPCR) assay for the detection of T. parva infections. However, the extent of sequence variation within this gene in the non-pathogenic Theileria spp. of the Africa buffalo is not well known. The aim of this study was, therefore, to characterize the full-length 18S rRNA genes of T. mutans, Theileria sp. (strain MSD) and T. velifera and to determine the possible influence of any sequence variation on the specific detection of T. parva using the 18S rRNA qPCR. The reverse line blot (RLB) hybridization assay was used to select samples which either tested positive for several different Theileria spp., or which hybridized only with the Babesia/Theileria genus-specific probe and not with any of the Babesia or Theileria speciesspecific probes. The full-length 18S rRNA genes from 14 samples, originating from 13 buffalo and one bovine from different localities in South Africa, were amplified, cloned and the resulting recombinants sequenced. Variations in the 18S rRNA gene sequences were identified in T. mutans, Theileria sp. (strain MSD) and T. velifera, with the greatest diversity observed amongst the T. mutans variants. This variation possibly explained why the RLB hybridization assay failed to detect T. mutans and T. velifera in some of the analysed samples. en
dc.description.librarian hb2013 en
dc.description.librarian ab2013
dc.description.sponsorship South African National Research Foundation (NRF ICD2006072000009) and UP Research Development Programme. en
dc.description.uri en
dc.identifier.citation Chaisi, ME, Collins, NE, Potgieter, FT & Oosthuizen MC 2013, 'Sequence variation identified in the 18S rRNA gene of Theileria mutans and Theileria velifera from the African buffalo (Syncerus caffer)', Veterinary Parasitology, vol. 191, no. 1-2, pp.132-137. en
dc.identifier.issn 0304-4017 (print)
dc.identifier.issn 1463-5224 (online)
dc.identifier.other 10.1016/j.vetpar.2012.08.005
dc.identifier.other 7004592997
dc.identifier.other 7103250386
dc.identifier.other N-8706-2014
dc.identifier.other O-6342-2014
dc.language.iso en en
dc.publisher Elsevier en
dc.relation.requires Adobe Acrobat Reader en
dc.rights © 2012 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Veterinary Parasitology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Veterinary Parasitology, vol. 191, issue 1-2, 2013, doi. 10.1016/j.vetpar.2012.08.005 en
dc.subject Syncerus caffer en
dc.subject Theileria mutans en
dc.subject Theileria velifera en
dc.subject Theileria sp. (strain MSD) en
dc.subject Reverse line blot hybridization assay en
dc.subject 18S rRNA gene en
dc.subject Phylogenetic analysis en
dc.subject.lcsh African buffalo (Syncerus caffer) en
dc.subject.lcsh Theileria en
dc.title Sequence variation identified in the 18S rRNA gene of Theileria mutans and Theileria velifera from the African buffalo (Syncerus caffer) en
dc.type Postprint Article en

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