dc.contributor.author |
Mans, Janet
|
|
dc.contributor.author |
Netshikewta, Rembuluwani
|
|
dc.contributor.author |
Magwalivha, Mpho
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dc.contributor.author |
Van Zyl, Walda B.
|
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dc.contributor.author |
Taylor, Maureen B.
|
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dc.date.accessioned |
2013-04-10T07:46:48Z |
|
dc.date.available |
2014-03-21T00:20:03Z |
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dc.date.issued |
2012-03-21 |
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dc.description.abstract |
This study aimed to assess norovirus (NoV) contamination and genotype diversity in surface
water in Gauteng, South Africa. Between January 2008 and December 2010, three rivers, namely
Klip, Suikerbosrant, and Rietspruit were monitored for NoV genogroup (G)I and GII. Viruses
were recovered using the glass wool adsorption-elution technique and detected by real-time
reverse transcription–polymerase chain reaction. From 2008 to 2010, NoVs were detected in 66%
(70/106) of Klip river samples. The Rietspruit and Suikerbosrant rivers were contaminated with
NoV in 95% (20/21) and 21% (5/24) of samples, respectively. NoV-positive samples comprised
of 33% GI, 29% GII and 38% of both GI and GII strains. Based on partial capsid gene analysis
(region C), 16 NoV genotypes (6 GI, 10 GII) were identified. The major genotypes detected were
GI.4, GI.5 and GII.4. These rivers could be a potential source of NoV infection for communities
using the water for domestic or recreational purposes. |
en |
dc.description.librarian |
am2013 |
en |
dc.description.librarian |
ab2013 |
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dc.description.sponsorship |
Dr Janet Mans was supported
by postdoctoral fellowships from the Poliomyelitis
Research Foundation (PRF), South Africa, and
the Claude Leon Foundation. Rembuluwani
Netshikweta and Mpho Magwalivha were supported
by postgraduate PRF bursaries. The study was
funded, in part, by the PRF (Grant no. 09/33, 2010)
and the Technology and Human Resources for
Industry Programme (THRIP Project no. 71896) and
NHLS Research Trust (Grant no. 94182) (2010–2011). |
en |
dc.description.uri |
http://journals.cambridge.org/action/displayJournal?jid=HYG |
en |
dc.identifier.citation |
Mans, J, Netshikweta, R, Magwalivha, M, Van Zyl, WB & Taylor, MB 2013, 'Diverse norovirus genotypes identified in sewage-polluted river water in South Africa', Epidemiology and Infection, vol. 141, no. 2, pp. 303-313. |
en |
dc.identifier.issn |
issn:0950-2688 (print) |
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dc.identifier.issn |
issn:1469-4409 (online |
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dc.identifier.other |
10.1017/S0950268812000490 |
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dc.identifier.uri |
http://hdl.handle.net/2263/21272 |
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dc.language.iso |
en |
en |
dc.publisher |
Cambridge University Press |
en |
dc.relation.requires |
Adobe Acrobat Reader |
en |
dc.rights |
© Cambridge University Press. |
en |
dc.subject |
Genotypes |
en |
dc.subject |
Norovirus |
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dc.subject |
River water |
en |
dc.subject |
South Africa |
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dc.subject.lcsh |
Water -- Microbiology |
en |
dc.subject.lcsh |
Viral pollution of water |
en |
dc.subject.lcsh |
Water -- Pollution |
en |
dc.title |
Diverse norovirus genotypes identified in sewage-polluted river water in South Africa |
en |
dc.type |
Article |
en |