Evolutionary dynamics of 2009 pandemic influenza A virus subtype H1N1 in South Africa during 2009–2010

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dc.contributor.author Venter, Marietjie
dc.contributor.author Naidoo, Dhamari
dc.contributor.author Pretorius, Marthi A.
dc.contributor.author Buys, Amelia
dc.contributor.author McAnerney, Johanna M.
dc.contributor.author Blumberg, Lucille H.
dc.contributor.author Madhi, Shabir A.
dc.contributor.author Cohen, Cheryl
dc.contributor.author Schoub, Barry D.
dc.date.accessioned 2013-02-14T12:31:41Z
dc.date.available 2013-12-31T00:20:05Z
dc.date.issued 2012-12
dc.description Presented in part: Africa Influenza Scientific Symposium, NCID, Johannesburg, South Africa, 7–11 December 2009; Options for the Control of Influenza, Hong Kong, SAR, 3–7 September 2010. en_US
dc.description.abstract BACKGROUND: 2009 Pandemic Influenza A(H1N1) was first detected in June 2009 in South Africa and later resulted in extensive transmission throughout Africa. Established routine surveillance programmes and collaboration between private and public sector laboratories allowed for comprehensive molecular epidemiological and antigenic investigation of the first and second waves of 2009 Pandemic Influenza A(H1N1) in South Africa. METHODS: We screened 9792 and 6915 specimens from patients with Influenza like illness (ILI) or severe acute respiratory infection (SARI) symptoms from surveillance programmes in hospitalised or outpatients in South Africa in 2009 and 2010, respectively for Influenza by RTPCR. Influenza-positive specimens were subjected to genetic and antigenic characterisation. Bayesian and Maximum likelihood analyses of the Hemaglutinin (HA)genes of 96 2009 Pandemic Influenza A(H1N1) strains were used for molecular epidemiological investigations. Hemaglutination inhibition assays and sequencing of the PB2 and neuraminidase genes were used to investigate pathogenicity and resistance mutations. RESULTS: The 2009 Pandemic Influenza A(H1N1) epidemic occurred as a second epidemic peak following seasonal H3N2 cases in 2009 and in 2010. Progressive drift away from the A/California/7/2009 vaccine strain was observed at both the nucleotide and amino acid level with 2010 strains clustering separate to 2009 strains. A few unique clusters of amino acid changes in severe cases were identified, but most strains were antigenically similar to the vaccine strain and no resistance or known pathogenicity mutations were detected. CONCLUSION: Despite limited drift observed over the 2 seasons in South Africa, circulating 2009 Pandemic Influenza A(H1N1) strains remained antigenically similar to strains identified in other Northern and Southern hemisphere countries from 2010 and 2011. en_US
dc.description.librarian hb2013 en_US
dc.description.librarian ay2013 en
dc.description.sponsorship Centers for Disease Control and Prevention, Atlanta, Georgia, USA. en_US
dc.description.uri http://www.journals.uchicago.edu/toc/jid/current en_US
dc.identifier.citation Venter, M, Naidoo, D, Pretorius, M, Buys, A, McAnerney, J, Blumberg, L, Madhi, SA, Cohen, C & Schoub, B 2012, 'Evolutionary dynamics of 2009 pandemic influenza A virus subtype H1N1 in South Africa during 2009–2010', Journal of Infectious Diseases, vol. 206, suppl.1, pp. S166-S172. en_US
dc.identifier.issn 0022-1899 (print)
dc.identifier.issn 1537-6613 (online)
dc.identifier.other 10.1093/infdis/jis539
dc.identifier.uri http://hdl.handle.net/2263/21015
dc.language.iso en en_US
dc.publisher Oxford University Press en_US
dc.rights © The Author 2012. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. en_US
dc.subject Pandemic H1N1 en_US
dc.subject South Africa en_US
dc.subject Molecular epidemiology en_US
dc.subject Pathogenic markers en_US
dc.subject Drift en_US
dc.subject.lcsh Influenza viruses en
dc.title Evolutionary dynamics of 2009 pandemic influenza A virus subtype H1N1 in South Africa during 2009–2010 en_US
dc.type Postprint Article en_US


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