Prevalence of Clostridium difficile toxin genes in Pretoria

Show simple item record Storm, Nadia Kock, Marleen M. Kruger, M.D. Ehlers, Marthie Magdaleen 2013-01-31T13:09:52Z 2013-01-31T13:09:52Z 2012
dc.description.abstract The hypervirulent polymerase chain reaction (PCR) ribotype 027 strain of Clostridium difficile produces toxins A, B and a binary toxin. Toxin detection kits are commonly used in diagnostic laboratories, but have been unsuccessful in detecting all of the relevant C. difficile strains, and the toxins produced. In this study, conventional PCR was used to detect the presence of the genes of toxin A, toxin B and the binary toxin of C. difficile. Eighty-four frozen (collected between 2006-2007) and 13 fresh (collected in 2010) stool specimens, obtained in Pretoria, were analysed. The genes for toxin A, toxin B and the binary toxin were detected in one of the fresh stool specimens. This may have implications for healthcare facilities, and suggests the possible emergence of the highly virulent PCR ribotype 027 strain of C. difficile in Pretoria. This emphasises the importance of continuous surveillance and monitoring of C. difficile outbreaks. en_US
dc.description.librarian ay2013 en
dc.description.uri en_US
dc.identifier.citation Storm, N, Kock, MM, Kruger, MD & Ehlers, MM 2012, 'Prevalence of Clostridium difficile toxin genes in Pretoria', Southern African Journal of Epidemiology & Infection, vol. 27 no. 4 pp. 164-168. en_US
dc.identifier.issn 1015-8782
dc.language.iso en en_US
dc.publisher Federation of Infectious Diseases Societies of South Africa en_US
dc.rights © SAJEI en_US
dc.subject Clostridium difficile en_US
dc.subject Toxin A en_US
dc.subject Toxin B en_US
dc.subject Binary toxin en_US
dc.subject Conventional PCR en_US
dc.subject.lcsh Clostridium diseases -- South Africa en
dc.subject.lcsh Diarrhea -- Diagnosis en
dc.title Prevalence of Clostridium difficile toxin genes in Pretoria en_US
dc.type Article en_US

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