2-Methoxyestradiol-bis-sulphamate refrains from inducing apoptosis and autophagy in a non-tumorigenic breast cell line

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dc.contributor.author Visagie, M.H. (Michelle Helen)
dc.contributor.author Joubert, Annie M.
dc.date.accessioned 2013-01-31T12:47:29Z
dc.date.available 2013-01-31T12:47:29Z
dc.date.issued 2012-08-20
dc.description.abstract BACKGROUND: Anticancer research resulted in the discovery of a promising antimitotic metabolite, 2-methoxyestradiol. 2-Methoxyestradiol-bis-sulphamate, a bis-sulphamoylated analogue exerts antiproliferative- and antimitotic activity. Investigating the anticancer potential of 2-methoxyestradiol-bis-sulphamate requires demonstrating the influence of 2-methoxyestradiol-bis-sulphamate on non-tumorigenic cells. This project focused on the in vitro effects of 2-methoxyestradiol-bis-sulphamate on the non-tumorigenic MCF-12A breast epithelial cell line. METHODS The in vitro influence of 2-methoxyestradiol-bis-sulphamate was investigated on cell cycle progression, possible induction of apoptosis and autophagy and reactive oxygen species generation. Cell cycle progression was done using flow cytometry in conjunction with ethanol fixation and propidium iodide staining. Displaying effects on the mitochondrial membrane potential was achieved utilizing flow cytometry and the MitoCapture TM Mitochondrial apoptosis detection kit. Autophagy detection was done by means of flow cytometry and anti-LC3B conjugated to DyLight 488. Reactive oxygen species generation was conducted employing flow cytometry and 2,7-dichlorofluorescein diacetate and hydroethidine. RESULTS: This study demonstrated that 2-methoxyestradiol-bis-sulphamate did not affect cell cycle progression or reactive oxygen species in a statistically significant manner in the non-tumorigenic MCF-12A cell line. In addition, 2-methoxyestradiol-bis-sulphamate did not statistically significantly induce apoptosis or autophagy. CONCLUSION: Reports indicate that 2-methoxyestradiol-bis-sulphamate induces apoptosis and autophagy in several tumorigenic cell lines. The anticancer ability of 2-methoxyestradiol-bis-sulphamate is due to its antimitotic activity. However, this study demonstrates the promising notion that 2-methoxyestradiol-bis-sulphamate does not affect the non-tumorigenic MCF-12A cells. This project contributes to the embedded scientific knowledge regarding the differential death mechanisms used by 2-methoxyestradiol-bis-sulphamate on tumorigenic and non-tumorigenic cell lines. en_US
dc.description.librarian am2013 en_US
dc.description.librarian ay2013 en
dc.description.uri http://www.cancerci.com/content/12/1/37 en_US
dc.identifier.citation Visagie and Joubert: 2-Methoxyestradiol-bissulphamate refrains from inducing apoptosis and autophagy in a non-tumorigenic breast cell line. Cancer Cell International 2012 12:37. en_US
dc.identifier.issn 1475-2867 (online)
dc.identifier.issn 1475-2867 (print)
dc.identifier.other 10.1186/1475-2867-12-37
dc.identifier.uri http://hdl.handle.net/2263/20913
dc.language.iso en en_US
dc.publisher BioMed Central en_US
dc.rights © 2012 Visagie and Joubert; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License en_US
dc.subject Apoptosis en_US
dc.subject Autophagy en_US
dc.subject Non-tumorigenic en_US
dc.subject Reactive oxygen species en_US
dc.subject 2-Methoxyestradiol-bis-sulphamate en
dc.subject.lcsh Antineoplastic agents -- Research en
dc.title 2-Methoxyestradiol-bis-sulphamate refrains from inducing apoptosis and autophagy in a non-tumorigenic breast cell line en_US
dc.type Article en_US


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