Comparison of ELISA and HI for detection of antibodies against Wesselsbron disease virus

Show simple item record Williams, R. Schoeman, M. Van Wyk, A. Roos, K. Josemans, E.J.
dc.contributor.editor Verwoerd, Daniel Wynand 2012-12-12T09:06:15Z 2012-12-12T09:06:15Z 2012 1997
dc.description The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en_US
dc.description.abstract A two-graph Receiver Operating Characteristic analysis was done to determine the optimal cut-off value of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Wesselsbron disease (WSL) virus. When ELISA and haemagglutination-inhibition (HI) results of WSL-positive and WSL-negative control sheep sera were compared, the sensitivity of ELISA was 97,9% and that of HI, 87,5%, while the specificity of ELISA and HI were 95,7% and 100 %, respectively. The ELISA's superior sensitivity was confirmed by the results of the two assays performed on a simulated range of low-positive sera, which showed that the ELISA was able to detect WSL-antibody levels at least ten times lower than those the HI could. The ELISA was also less cross-reactive than the HI to guinea-pig antisera against nine flaviviruses relevant to southern Africa. The combination of the ELISA's ability to test untreated sera in single dilution and its superior sensitivity and lower crossreactivity as compared to that of the traditional HI, makes it the assay of choice for automation and large-scale screening of animals for antibodies to WSL virus. en_US
dc.identifier.citation Williams, R, Schoeman, M, Van Wyk, A, Roos, K & Josemans, EJ 1997, 'Comparison of ELISA and HI for detection of antibodies against Wesselsbron disease virus’. Onderstepoort Journal of Veterinary Research, vol. 64, no. 4, pp. 245-250. en_US
dc.identifier.issn 0330-2465
dc.language.iso en en_US
dc.publisher Published by the Agricultural Research Council, Onderstepoort Veterinary Institute en_US
dc.rights © ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital). en_US
dc.subject Veterinary medicine en_US
dc.subject Antibodies en_US
dc.subject Comparison en_US
dc.subject Detection of antibodies en_US
dc.subject ELISA en_US
dc.subject Enzyme-linked immunosorbent assay en_US
dc.subject Haemagglutination inhibition en_US
dc.subject HI en_US
dc.subject Wesselsbron disease virus en_US
dc.subject WSL en_US
dc.subject.lcsh Veterinary medicine -- South Africa
dc.title Comparison of ELISA and HI for detection of antibodies against Wesselsbron disease virus en_US
dc.type Article en_US

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