Comparison of indirect fluorescent antibody test and enzyme linked immunosorbent assay in the detection of exposure of cattle to Theileria parva in Kenya

Abstract

Appraisal of the indirect fluorescent antibody test (IFAT) and antigen enzyme linked immunosorbent assay (ELISA) serological tests as carried out to detect cattle exposed to Theileria parva at the National Veterinary Research Centre, Muguga (NVRC), Kenya is reported. Using sera from T parva naive cattle and cattle experimentally exposed to T parva, the two tests were appraised in terms of their sensitivity and specificity.IFAT and ELISA had the same sensitivity of 90% while ELISA had a higher specificity (90 %) than IFAT (80 %). A comparison was also made of the capability of the two tests to detect exposure of dairy cattle to T parva prior to immunization against East Coast fever (ECF). The positive outcome from the IFAT was significantly higher (X2 = 30,36; P < 0,001) than that from the ELISA. The agreement between the two tests was low (Kappa= 0,21 ). The two tests indicated a higher risk of ECF in the study area than was expected. Indications are that the ELISA has been effectively adopted at NVRC.