Inactivation of Wolbachia reveals its biological roles in whitefly host

Show simple item record Xue, Xia Li, Shao-Jian Ahmed, Muhammad Zaheerudin De Barro, P.J. (Paul Joseph), 1963- Ren, Shun-Xiang Qiu, Bao-Li
dc.contributor.editor Zilberstein, Dan 2012-11-28T07:15:41Z 2012-11-28T07:15:41Z 2012-10-29
dc.description.abstract BACKGROUND: The whitefly Bemisia tabaci is cryptic species complex composed of numerous species. Individual species from the complex harbor a diversity of bacterial endosymbionts including Wolbachia. However, while Wolbachia is known to have a number of different roles, its role in B. tabaci is unclear. Here, the antibiotic rifampicin is used to selectively eliminate Wolbachia from B. tabaci so as to enable its roles in whitefly development and reproduction to be explored. The indirect effects of Wolbachia elimination on the biology of Encarsia bimaculata, a dominant parasitoid of B. tabaci in South China, were also investigated. METHODOLOGY/PRINCIPAL FINDING: qRT-PCR and FISH were used to show that after 48 h exposure to 1.0 mg/ml rifampicin, Wolbachia was completely inactivated from B. tabaci Mediterranean (MED) without any significant impact on either the primary symbiont, Portiera aleyrodidarum or any of the other secondary endosymbionts present. For B. tabaci MED, Wolbachia was shown to be associated with decreased juvenile development time, increased likelihood that nymphs completed development, increased adult life span and increased percentage of female progeny. Inactivation was associated with a significant decrease in the body size of the 4th instar which leads us to speculate as to whether Wolbachia may have a nutrient supplementation role. The reduction in nymph body size has consequences for its parasitoid, E. bimaculata. The elimination of Wolbachia lead to a marked increase in the proportion of parasitoid eggs that completed their development, but the reduced size of the whitefly host was also associated with a significant reduction in the size of the emerging parasitoid adult and this was in turn associated with a marked reduction in adult parasitoid longevity. CONCLUSIONS/SIGNIFICANCE: Wolbachia increases the fitness of the whitefly host and provides some protection against parasitization. These observations add to our understanding of the roles played by bacterial endosymbionts. en_US
dc.description.sponsorship This research was funded by the National Basic Research Program of China (2009CB119203), the Program for New Century Excellent Talents in University (NCET-0917–2011) and the China National Natural Science Foundation (31071732). en_US
dc.description.uri en_US
dc.identifier.citation Xue X, Li S-J, Ahmed MZ, De Barro PJ, Ren S-X, et al. (2012) Inactivation of Wolbachia Reveals Its Biological Roles in Whitefly Host. PLoS ONE 7(10): e48148. DOI:10.1371/journal.pone.0048148. en_US
dc.identifier.issn 1932-6203
dc.identifier.other 10.1371/journal.pone.0048148
dc.language.iso en en_US
dc.publisher Public Library of Science en_US
dc.rights © 2012 Xue et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License en_US
dc.subject Whitefly en_US
dc.subject Bemisia tabaci en_US
dc.subject Wolbachia en_US
dc.title Inactivation of Wolbachia reveals its biological roles in whitefly host en_US
dc.type Article en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record