Virological and serological findings in Rousettus aegyptiacus experimentally inoculated with vero cells-adapted hogan strain of Marburg virus

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dc.contributor.author Paweska, Janusz Tadeusz
dc.contributor.author Jansen van Vuren, Petrus
dc.contributor.author Masumu, Justin
dc.contributor.author Leman, Patricia A.
dc.contributor.author Grobbelaar, Antoinette A.
dc.contributor.author Birkhead, Monica
dc.contributor.author Clift, Sarah J.
dc.contributor.author Swanepoel, Robert
dc.contributor.author Kemp, Alan
dc.contributor.editor Markotter, Wanda
dc.date.accessioned 2012-11-27T05:58:42Z
dc.date.available 2012-11-27T05:58:42Z
dc.date.issued 2012-09-17
dc.description.abstract The Egyptian fruit bat, Rousettus aegyptiacus, is currently regarded as a potential reservoir host for Marburg virus (MARV). However, the modes of transmission, the level of viral replication, tissue tropism and viral shedding pattern remains to be described. Captive-bred R. aegyptiacus, including adult males, females and pups were exposed to MARV by different inoculation routes. Blood, tissues, feces and urine from 9 bats inoculated by combination of nasal and oral routes were all negative for the virus and ELISA IgG antibody could not be demonstrated for up to 21 days post inoculation (p.i.). In 21 bats inoculated by a combination of intraperitoneal/subcutaneous route, viremia and the presence of MARV in different tissues was detected on days 2–9 p.i., and IgG antibody on days 9–21 p.i. In 3 bats inoculated subcutaneously, viremia was detected on days 5 and 8 (termination of experiment), with virus isolation from different organs. MARV could not be detected in urine, feces or oral swabs in any of the 3 experimental groups. However, it was detected in tissues which might contribute to horizontal or vertical transmission, e.g. lung, intestines, kidney, bladder, salivary glands, and female reproductive tract. Viremia lasting at least 5 days could also facilitate MARV mechanical transmission by blood sucking arthropods and infections of susceptible vertebrate hosts by direct contact with infected blood. All bats were clinically normal and no gross pathology was identified on post mortem examination. This work confirms the susceptibility of R. aegyptiacus to infection with MARV irrespective of sex and age and contributes to establishing a bat-filovirus experimental model. Further studies are required to uncover the mode of MARV transmission, and to investigate the putative role of R. aegyptiacus as a reservoir host. en
dc.description.librarian mn2012 en
dc.description.sponsorship The Wellcome Trust grant WT087546MA under its African Institutions Initiative (http:// www.wellcome.ac.uk/Funding/International/WTX055734.htm en
dc.description.uri http://www.plosone.org en
dc.identifier.citation Paweska JT, Jansen van Vuren P, Masumu J, Leman PA, Grobbelaar AA, et al. (2012) Virological and Serological Findings in Rousettus aegyptiacus Experimentally Inoculated with Vero Cells-Adapted Hogan Strain of Marburg Virus. PLoS ONE 7(9): e45479. DOI:10.1371/journal.pone.0045479. en
dc.identifier.issn 1932-6203
dc.identifier.other 10.1371/journal.pone.0045479
dc.identifier.uri http://hdl.handle.net/2263/20494
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2012 Paweska et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, en
dc.subject Egyptian fruit bat en
dc.subject Rousettus aegyptiacus en
dc.subject Marburg virus en
dc.subject MARV
dc.subject.lcsh Veterinary virology en
dc.title Virological and serological findings in Rousettus aegyptiacus experimentally inoculated with vero cells-adapted hogan strain of Marburg virus en
dc.type Article en


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