Physicochemical modification of kafirin microparticles and their ability to bind bone morphogenetic protein-2 (BMP-2), for application as a biomaterial
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Date
Authors
Anyango, Joseph Ochieng
Duneas, Nicolaas
Taylor, J.R.N. (John Reginald Nuttall)
Taylor, Janet
Journal Title
Journal ISSN
Volume Title
Publisher
American Chemical Society
Abstract
Vacuolated spherical kafirin microparticles with a mean diameter of 5 μm can be formed from an acidic solution
with water addition. Three-dimensional scaffolds for hard tissue repair require large structures with a high degree of
interconnected porosity. Cross-linking the formed kafirin microparticles using wet heat or glutaraldehyde treatment resulted in
larger structures (approximately 20 μm), which, while similar in size and external morphology, were apparently formed by further
assisted assembly by two significantly different mechanisms. Heat treatment, which increased the vacuole size, involved kafirin
polymerization by disulfide bonding with the microparticles being formed from round, coalesced nanostructures, as shown by
atomic force microscopy (AFM). Kafirin polymerization of glutaraldehyde-treated microparticles was not by disulfide bonding,
and the nanostructures, as revealed by AFM, were spindle shaped. Both treatments enhanced BMP-2 binding to the
microparticles, probably due to their increased size. Thus, these modified kafirin microparticles have potential as natural,
nonanimal protein bioactive scaffolds.
Description
Keywords
Kafirin, Microparticle, Cross-linking, Binding, BMP-2
Sustainable Development Goals
Citation
Anyango, JO, Duneas, N, Taylor, JRN & Taylor, J 2012, 'Physicochemical modification of kafirin microparticles and their ability to bind bone morphogenetic protein-2 (BMP-2), for application as a biomaterial', Journal of Agricultural and Food Chemistry, vol. 60, no. 34, pp. 8419-8426.