dc.contributor.author |
Anyango, Joseph Ochieng
|
|
dc.contributor.author |
Duneas, Nicolaas
|
|
dc.contributor.author |
Taylor, J.R.N. (John Reginald Nuttall)
|
|
dc.contributor.author |
Taylor, Janet
|
|
dc.date.accessioned |
2012-09-26T11:37:09Z |
|
dc.date.available |
2013-08-31T00:20:04Z |
|
dc.date.issued |
2012-08 |
|
dc.description.abstract |
Vacuolated spherical kafirin microparticles with a mean diameter of 5 μm can be formed from an acidic solution
with water addition. Three-dimensional scaffolds for hard tissue repair require large structures with a high degree of
interconnected porosity. Cross-linking the formed kafirin microparticles using wet heat or glutaraldehyde treatment resulted in
larger structures (approximately 20 μm), which, while similar in size and external morphology, were apparently formed by further
assisted assembly by two significantly different mechanisms. Heat treatment, which increased the vacuole size, involved kafirin
polymerization by disulfide bonding with the microparticles being formed from round, coalesced nanostructures, as shown by
atomic force microscopy (AFM). Kafirin polymerization of glutaraldehyde-treated microparticles was not by disulfide bonding,
and the nanostructures, as revealed by AFM, were spindle shaped. Both treatments enhanced BMP-2 binding to the
microparticles, probably due to their increased size. Thus, these modified kafirin microparticles have potential as natural,
nonanimal protein bioactive scaffolds. |
en_US |
dc.description.sponsorship |
University of Pretoria
Postgraduate Research Support Bursary |
en_US |
dc.description.uri |
http://pubs.acs.org/JAFC |
en_US |
dc.identifier.citation |
Anyango, JO, Duneas, N, Taylor, JRN & Taylor, J 2012, 'Physicochemical modification of kafirin microparticles and their ability to bind bone morphogenetic protein-2 (BMP-2), for application as a biomaterial', Journal of Agricultural and Food Chemistry, vol. 60, no. 34, pp. 8419-8426. |
en_US |
dc.identifier.issn |
0021-8561 (print) |
|
dc.identifier.issn |
1520-5118 (online) |
|
dc.identifier.other |
10.1021/jf302533e |
|
dc.identifier.uri |
http://hdl.handle.net/2263/19896 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
American Chemical Society |
en_US |
dc.rights |
© 2012 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, copyright American Chemical Society after peer review and technical editing by the publisher. |
en_US |
dc.subject |
Kafirin |
en_US |
dc.subject |
Microparticle |
en_US |
dc.subject |
Cross-linking |
en_US |
dc.subject |
Binding |
en_US |
dc.subject |
BMP-2 |
en_US |
dc.title |
Physicochemical modification of kafirin microparticles and their ability to bind bone morphogenetic protein-2 (BMP-2), for application as a biomaterial |
en_US |
dc.type |
Postprint Article |
en_US |