Effect of suppressing the synthesis of different kafirin subclasses on grain endosperm texture, protein body structure and protein nutritional quality in improved sorghum lines
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Date
Authors
Da Silva, Laura S.
Jung, Rudolf
Zhao, Zuo-yo
Glassman, Kimberly
Taylor, Janet
Taylor, J.R.N. (John Reginald Nuttall)
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
To improve sorghum grain protein nutritional quality, improved sorghum lines were transformed to
suppress the synthesis of different kafirin sub-classes, or backcrossed into transgenic lines with
improved protein quality. Co-suppression of the alpha-, gamma- and delta-kafirin sub-classes and
removal of the tannin trait resulted in transgenic sorghum lines with high cooked protein digestibility
( 80%), improved Amino Acid Score (0.8) and Protein Digestibility Corrected Amino Acid Score (0.7)
compared to the non-transgenic null controls ( 50%, 0.4 and 0.2, respectively). These high protein
quality lines had a floury endosperm. They also had modified protein body structure, where the protein
bodies were irregular shaped with few to numerous invaginations and were less densely packed, with
a dense protein matrix visible around the protein bodies. When fewer sub-classes were suppressed, i.e.
gamma 1 and delta 2, the endosperm was corneous with normal protein body structure but the
improvement in cooked protein digestibility appeared to be less. Apparently, co-suppression of several
kafirin sub-classes is required to obtain high protein nutritional quality sorghum lines, but this seems to
result in floury-type grain endosperm texture.
Description
Keywords
Sorghum, Transgenic, Protein bodies, Digestibility
Sustainable Development Goals
Citation
Laura S. da Silva, Rudolf Jung, Zhu-yo Zhao, Kimberly Glassman, Janet Taylor & John R.N. Taylor, Effect of suppressing the synthesis of different kafirin subclasses on grain endosperm texture, protein body structure and protein nutritional quality in improved sorghum lines, Journal of Cerial Science, vol. 54, no. 1, pp. 160-167 (2011), doi: 10.1016/j.jcs.2011.04.009.