The exposure and response of the catfish, Clarias gariepinus, was studied in male specimens collected in the vicinity of a DDT spraying programme to control malaria. Two sites were situated in the DDT sprayed areas and one site upstream from exposed areas, used as a reference site the collected specimens were analysed for DDT bioaccumulation and the extent of associated effects. The concentration of all DDT metabolites including p,p’ –and o,p’ –forms of DDT, DDE and DDD, were measured in the adipose tissue, whilst the effects were measured using a range of biomarkers. This included assessing the effectiveness of plasma calcium, magnesium, zinc and alkali-labile phosphates (ALPs) as indirect measures of vitellogenin (VTG). Gonad condition was determined by calculating the gonadosomatic index (GIS) for each individual and comparing it with the gonad mass that were adjusted with Analysis of Covariance (ANCOVA). The presence of intersex in gonads was identified and the overall body condition determined using the condition factor (CF). Overall, none of the biomarkers showed significant change in the presence of high levels of DDT nor lindane, dieldrin and endosulfan II. Subtle responses is the plasma concentrations of calcium, ALP and gonad condition were evident in the catfish where DDT concentrations were highest, whilst no effects related to intersex and body condition were evident. Overall this study highlighted the tolerance of C. gariepinus to DDT contamination, the practical implications of using biomarkers in developing countries, and the need for further research into developing biomarkers for much needed biomonitoring programmes in areas where malaria control programmes continue to use DDT.