Pantoea allii sp.nov., isolated from onion plants and seed

Show simple item record Brady, Carrie Louise Goszczynska, Teresa Venter, S.N. (Stephanus Nicolaas) Cleenwerck, Ilse De Vos, Paul Gitaitis, Ronald D. Coutinho, Teresa A. 2012-07-04T08:47:52Z 2012-07-04T08:47:52Z 2011
dc.description.abstract Eight yellow-pigmented, Gram-negative, rod-shaped, oxidase-negative, motile, facultatively anaerobic bacteria were isolated from onion seed in South Africa and from an onion plant exhibiting centre rot symptoms in the USA. The isolates were assigned to the genus Pantoea on the basis of phenotypic and biochemical tests. 16S rRNA gene sequence analysis and multilocus sequence analysis (MLSA), based on gyrB, rpoB, infB and atpD sequences, confirmed the allocation of the isolates to the genus Pantoea. MLSA further indicated that the isolates represented a novel species, which was phylogenetically most closely related to Pantoea ananatis and Pantoea stewartii. Amplified fragment length polymorphism analysis also placed the isolates into a cluster separate from P. ananatis and P. stewartii. Compared with type strains of species of the genus Pantoea that showed .97% 16S rRNA gene sequence similarity with strain BD 390T, the isolates exhibited 11–55% whole-genome DNA–DNA relatedness, which confirmed the classification of the isolates in a novel species. The most useful phenotypic characteristics for the differentiation of the isolates from their closest phylogenetic neighbours are production of acid from amygdalin and utilization of adonitol and sorbitol. A novel species, Pantoea allii sp. nov., is proposed, with type strain BD 390T (5LMG 24248T). en
dc.description.librarian nf2012 en
dc.description.sponsorship The South African–Flemish Bilateral Agreement, the Agricultural Research Council (ARC), the National Research Foundation (NRF) and the THRIP support programme of the Department of Trade and Industry, South Africa. en_US
dc.description.uri en_US
dc.identifier.citation Brady, CL, Goszczynska, T, Venter, SN, Cleenwerck, I, De Vos, P, Gitaitis, RD & Coutinho, TA 2011, 'Pantoea allii sp.nov., isolated from onion plants and seed', International Journal of Systematic and Evolutionary Microbiology, vol. 61, no. 4, pp. 932-937. en
dc.identifier.issn 1466-5026 (print)
dc.identifier.issn 1466-5034 (online)
dc.identifier.other 10.1099/ijs.0.022921-0
dc.language.iso en en_US
dc.publisher Society for General Microbiology en_US
dc.rights © 2011 IUMS en_US
dc.subject Multilocus sequence analysis (MLSA) en
dc.subject Onion plants en
dc.subject Onion seed en
dc.subject Pantoea allii sp.nov en
dc.subject.lcsh DNA fingerprinting en
dc.subject.lcsh Onions -- Diseases and pests en
dc.subject.lcsh Anaerobic bacteria -- Genetics en
dc.title Pantoea allii sp.nov., isolated from onion plants and seed en
dc.type Postprint Article en

Files in this item

This item appears in the following Collection(s)

Show simple item record