Please be advised that the site will be down for maintenance on Sunday, September 1, 2024, from 08:00 to 18:00, and again on Monday, September 2, 2024, from 08:00 to 09:00. We apologize for any inconvenience this may cause.
dc.contributor.author | Korsman, J. | |
dc.contributor.author | Meisel, B. | |
dc.contributor.author | Kloppers, Frederik J. | |
dc.contributor.author | Crampton, Bridget Genevieve | |
dc.contributor.author | Berger, David Kenneth | |
dc.date.accessioned | 2012-06-01T08:26:23Z | |
dc.date.available | 2012-06-01T08:26:23Z | |
dc.date.issued | 2012 | |
dc.description.abstract | Grey leaf spot is an important maize foliar disease caused by the fungal pathogens Cercospora zeae-maydis and Cercospora zeina. Although methods exist to detect these Cercospora species in maize, current techniques do not allow quantification of the fungi in planta. We developed a real-time SYBR® Green PCR assay for quantification of grey leaf spot disease in maize based on the amplification of a fragment of a cytochrome P450 reductase (cpr1) gene. In planta fungal DNA content was normalised to a maize glutathione S-transferase III gene (gst3) to yield values was specific to the two Cercospora spp., and we observed no amplification of the cpr1 fragment in non-target maize leaf pathogens or saprophytes. The assay was employed to quantify C. zeina in glasshouse inoculated maize plants and grey leaf spot infected field plants of resistant and susceptible maize lines. In both instances, C. zeina DNA content correlated with symptomatic leaf lesion area, and the susceptible maize line contained significantly more C. zeina DNA than the resistant line. Sequence differences between the C. zeina and C. zeae-maydis cpr1 amplicons enabled us to perform melt curve analyses to identify the Cercospora species causing grey leaf spot at a particular location. This assay has application in the early detection and quantification of Cercospora spp., both of which are important tools in grey leaf spot disease management and maize breeding programmes. | en |
dc.description.librarian | nf2012 | en |
dc.description.sponsorship | The Technology Innovation Agency (TIA), the National Research Foundation (NRF) and the University of Pretoria (UP), South Africa. | en_US |
dc.description.uri | http://www.springerlink.com /content/100265/ | en_US |
dc.identifier.citation | Korsman, J, Meisel, B, Kloppers, FJ, Crampton, BG & Berger, DK 2012, 'Quantitative phenotyping of grey leaf spot disease in maize using real-time PCR', European Journal of Plant Pathology, vol. 133, pp. 461-471. | en |
dc.identifier.issn | 0929-1873 (print) | |
dc.identifier.issn | 1573-8469 (online) | |
dc.identifier.other | 10.1007/s10658-011-9920-1 | |
dc.identifier.uri | http://hdl.handle.net/2263/19060 | |
dc.language.iso | en | en_US |
dc.publisher | Springer | en_US |
dc.rights | © The Author(s) 2011. This article is published with open access at Springerlink.com. | en_US |
dc.subject | Cercospora zeae-maydis | en |
dc.subject | Cercospora zeina | en |
dc.subject | Precision phenotyping | en |
dc.subject | Quantitative polymerase chain reaction (qPCR) | en |
dc.subject | Grey leaf spot | en |
dc.subject | Real-time PCR | en |
dc.subject | PCR (Biochemistry) | en |
dc.subject.lcsh | Polymerase chain reaction -- Diagnostic use | en |
dc.subject.lcsh | Corn -- Diseases and pests -- Identification | en |
dc.subject.lcsh | Fungal diseases of plants -- Control | en |
dc.title | Quantitative phenotyping of grey leaf spot disease in maize using real-time PCR | en |
dc.type | Article | en |