In vitro chemo-preventative activity of Crotalaria agatiflora subspecies agatiflora Schweinf

Show simple item record Le Roux, Karlien Hussein, Ahmed A. Lall, Namrita 2012-05-28T06:38:45Z 2012-05-28T06:38:45Z 2011-12
dc.description.abstract ETHNOPHARMACOLOGICAL RELEVANCE: Crotalaria species have been widely used in Chinese traditional medicine to treat several types of internal cancers. Crotalaria agatiflora is used as a medicinal plant in several African countries for the treatment of bacterial and viral infections as well as for cancer. MATERIALS AND METHODS: Water and ethanol extracts of the leaves of Crotalaria agatiflora were evaluated for cytotoxicity on four cancerous and one noncancerous cell lines, using XTT (Sodium 3 -[1-(phenyl aminocarbonyl)- 3,4-tetrazolium]-bis-[4-methoxy-6-nitro] benzene sulfonic acid hydrate) colorimetric assay. Antioxidant activity was determined using DPPH (1,1-diphenyl-2-picryl hydrazyl). Light microscopy (eosin and haematoxylin staining) and flow cytometry (Annexin-V and propidium iodide) were used to evaluate the mechanism of action of the ethanol extract and one of the isolated compounds. RESULTS: The 50% inhibitory concentration (IC50) of the ethanol extract was found to be 73.9 g/mL against leukemic U-937 cells. Good antioxidant activity (IC50 = 18.89 g/mL) of the ethanol extract indicated the potential of Crotalaria agatiflora as chemo-preventative supplement. A bioassay guided fractionation of the ethanol extract led to the isolation of two pure compounds, namely madurensine and doronenine. Madurensine and doronenine showed moderate cytotoxicity on cancerous U-937 cells (IC50 values: 47.97 and 29.57 M respectively). The crude extract treated U-937 cells showed definite signs of cell death during light microscopic investigation, while little apoptosis (10–20%) and necrosis (<2%) were detected in cells treated with the extract or madurensine. CONCLUSIONS: The results indicated that Crotalaria agatiflora possesses potential chemopreventative and therapeutic properties. The exact mechanism of action should still be determined in future studies. It is hypothesized that the ethanolic extract as well as madurensine induces autophagy, which in prolonged circumstances may lead to autophagic cell death. en
dc.description.librarian nf2012 en
dc.description.uri en_US
dc.identifier.citation Karlien le Roux, Ahmed A. Hussein, Namrita Lall, In vitro chemo-preventative activity of Crotalaria agatiflora subspecies agatiflora Schweinf, Journal of Ethnopharmacology, vol. 138, no. 3, pp. 748-755 (2011), doi: 10.1016/j.jep.2011.10.011 en
dc.identifier.issn 0378-8741 (print)
dc.identifier.issn 1872-7573 (online)
dc.identifier.other 10.1016/j.jep.2011.10.011
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.rights © 2011 Elsevier Ireland Ltd. All rights reserved. en_US
dc.subject Crotalaria agatiflora en
dc.subject Cytotoxicity en
dc.subject Radical scavenging en
dc.subject.lcsh Crotalaria -- Therapeutic use en
dc.subject.lcsh Medicinal plants -- Africa en
dc.subject.lcsh Cancer -- Chemoprevention en
dc.subject.lcsh Flow cytometry -- Diagnostic use en
dc.title In vitro chemo-preventative activity of Crotalaria agatiflora subspecies agatiflora Schweinf en
dc.type Postprint Article en

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