SND2, a NAC transcription factor gene, regulates genes involved in secondary cell wall development in Arabidopsis fibres and increases fibre cell area in Eucalyptus

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dc.contributor.author Hussey, S.G. (Steven)
dc.contributor.author Mizrachi, Eshchar
dc.contributor.author Spokevicius, Antanas V.
dc.contributor.author Bossinger, Gerd
dc.contributor.author Berger, David Kenneth
dc.contributor.author Myburg, Alexander Andrew
dc.date.accessioned 2012-05-17T11:14:32Z
dc.date.available 2012-05-17T11:14:32Z
dc.date.issued 2011-12-01
dc.description.abstract BACKGROUND: NAC domain transcription factors initiate secondary cell wall biosynthesis in Arabidopsis fibres and vessels by activating numerous transcriptional regulators and biosynthetic genes. NAC family member SND2 is an indirect target of a principal regulator of fibre secondary cell wall formation, SND1. A previous study showed that overexpression of SND2 produced a fibre cell-specific increase in secondary cell wall thickness in Arabidopsis stems, and that the protein was able to transactivate the cellulose synthase8 (CesA8) promoter. However, the full repertoire of genes regulated by SND2 is unknown, and the effect of its overexpression on cell wall chemistry remains unexplored. RESULTS: We overexpressed SND2 in Arabidopsis and analyzed homozygous lines with regards to stem chemistry, biomass and fibre secondary cell wall thickness. A line showing upregulation of CesA8 was selected for transcriptome-wide gene expression profiling. We found evidence for upregulation of biosynthetic genes associated with cellulose, xylan, mannan and lignin polymerization in this line, in agreement with significant coexpression of these genes with native SND2 transcripts according to public microarray repositories. Only minor alterations in cell wall chemistry were detected. Transcription factor MYB103, in addition to SND1, was upregulated in SND2-overexpressing plants, and we detected upregulation of genes encoding components of a signal transduction machinery recently proposed to initiate secondary cell wall formation. Several homozygous T4 and hemizygous T1 transgenic lines with pronounced SND2 overexpression levels revealed a negative impact on fibre wall deposition, which may be indirectly attributable to excessive overexpression rather than cosuppression. Conversely, overexpression of SND2 in Eucalyptus stems led to increased fibre cross-sectional cell area. CONCLUSIONS: This study supports a function for SND2 in the regulation of cellulose and hemicellulose biosynthetic genes in addition of those involved in lignin polymerization and signalling. SND2 seems to occupy a subordinate but central tier in the secondary cell wall transcriptional network. Our results reveal phenotypic differences in the effect of SND2 overexpression between woody and herbaceous stems and emphasize the importance of expression thresholds in transcription factor studies. en
dc.description.librarian nf2012 en
dc.description.uri http://www.biomedcentral.com/1471-2229/11/173 en_US
dc.identifier.citation Hussey et al.: SND2, a NAC transcription factor gene, regulates genes involved in secondary cell wall development in Arabidopsis fibres and increases fibre cell area in Eucalyptus. BMC Plant Biology 2011 11:173. en
dc.identifier.issn 1471-2229 (print)
dc.identifier.issn 1471-2229 (online)
dc.identifier.other 10.1186/1471-2229-11-173
dc.identifier.uri http://hdl.handle.net/2263/18775
dc.language.iso en en_US
dc.rights © 2011 Hussey et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en_US
dc.subject Arabidopsis fibres en
dc.subject NAC transcription factor gene en
dc.subject Secondary cell wall development en
dc.subject SND2 en
dc.subject.lcsh Transcription factors en
dc.subject.lcsh Genetic transcription -- Regulation en
dc.subject.lcsh Biosynthesis en
dc.subject.lcsh Eucalyptus -- Genetic engineering en
dc.title SND2, a NAC transcription factor gene, regulates genes involved in secondary cell wall development in Arabidopsis fibres and increases fibre cell area in Eucalyptus en
dc.type Article en


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