A chemically defined medium for the growth of Cowdria ruminantium

Josemans, A.I.; Zweygarth, Erich

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dc.contributor.author	Josemans, A.I.
dc.contributor.editor	Boomker, Jacob Diederik Frederik
dc.contributor.upauthor	Zweygarth, Erich
dc.date.accessioned	2012-03-20T12:13:57Z
dc.date.available	2012-03-20T12:13:57Z
dc.date.created	2012
dc.date.issued	2001
dc.description	The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.	en
dc.description.abstract	Chemically defined media, termed SFMC-23 and SFMC-36, were devised for the in vitro culture of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants. Both media were based on Dulbecco's modified Eagle's medium nutrient mixture Ham F-12 (DME/F-12) containing various supplements. Medium SFMC-23 and SFMC-36 supported the long-term growth of the Welgevonden stock of C. ruminantium for a total of 55 and 28 passages, respectively, with regular passage intervals of 3 days. Using SFMC-23, split ratios varied from 5-10, depending on which host cell line was used. Other stocks of C. ruminantium (Sankat, Blaauwkrantz, Senegal) were successfully propagated for a test period of ten passages.	en
dc.description.librarian	mn2012	en
dc.description.sponsorship	Agricultural Research Council of South Africa. European Union (Cowdriosis Network) Grant no. IC18-CT95-0008 (DG12-SNRD).	en
dc.identifier.citation	Zweygarth, E & Josemans, AI 2001, 'A chemically defined medium for the growth of Cowdria ruminantium’. Onderstepoort Journal of Veterinary Research, vol. 68, no. 1, pp. 37-40.	en
dc.identifier.issn	0030-2465
dc.identifier.uri	http://hdl.handle.net/2263/18484
dc.language	en
dc.publisher	Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria.	en
dc.rights	© ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital).	en
dc.subject	Veterinary medicine	en
dc.subject	Chemically defined medium	en
dc.subject	Cowdria ruminantium	en
dc.subject	In vitro culture	en
dc.subject.lcsh	Veterinary medicine -- South Africa
dc.subject.lcsh	Heartwater -- South Africa	en
dc.subject.lcsh	Tick-borne diseases in animals	en
dc.title	A chemically defined medium for the growth of Cowdria ruminantium	en
dc.type	Article	en