Molecular analysis of bovine viral diarrhoea virus isolates from South Africa

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dc.contributor.author Kabongo, N.
dc.contributor.author Baule, C.
dc.contributor.editor Boomker, Jacob Diederik Frederik
dc.contributor.upauthor Van Vuuren, Moritz
dc.date.accessioned 2012-03-20T11:21:06Z
dc.date.available 2012-03-20T11:21:06Z
dc.date.created 2011
dc.date.issued 2003
dc.description The articles have been scanned with a HP Scanjet 8300; 600dpi, saved in TIFF format. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en
dc.description.abstract The presence of bovine viral diarrhoea virus in South Africa has been confirmed by several serological surveys. However, little is known about its biological properties. Twenty five isolates obtained by isolation in tissue culture and detected by means of the antigen capture ELISA from clinically sick cattle and from foetal calf serum in South Africa were characterized on the basis of analysis of the 5' non-translated (NTR) region of the genome. A reverse-transcription polymerase chain reaction (RT -PCR) was used to amplify specific sequences from the 5'NTR of the genome. The oligonucleotide primers corresponding to positions 105-125 and 399-378, respectively, in the sequence of BVDV strain NADL were used to generate the PCR products. Both strands were sequenced directly with these primers and fluorescence-labelled dideoxynucleotides in an automated nucleic acid sequencer. Reference strains of pestiviruses [(BVDV type I, BVDV type II, border disease virus (BDV) and hog cholera virus (HCV)] and isolates from a previous investigation on BVDV in southern Africa were included for comparative purposes. All the BVDV strains obtained during this study belong to subgroups of BVDV genotype I. No association could be demonstrated between the geographic origin of the isolates. A number of isolates formed another branch separate from the existing branches la, Ib and Ic. These findings suggest that extensive genetic diversity can be found within BVDV type I isolates from southern Africa. Isolates that group with the classical BVDV type I strains, particularly of American origin, coexist with variants that appear to represent a local genetic pool and or variants evolving from the classical strains. en
dc.description.librarian mn2012 en
dc.identifier.citation Kabongo, N, Baule, C & Van Vuuren, M 2003, 'Molecular analysis of bovine viral diarrhoea virus isolates from South Africa'. Onderstepoort Journal of Veterinary Research, vol. 70, no. 4, pp. 273-279. en
dc.identifier.issn 0030-2465
dc.identifier.other 7004572625
dc.identifier.other N-9080-2014
dc.identifier.uri http://hdl.handle.net/2263/18480
dc.language.iso en en
dc.publisher Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria. en
dc.rights © ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital). en
dc.subject Veterinary medicine en
dc.subject BVDV 5'NTR en
dc.subject Phylogeny en
dc.subject Reverse-transcription polymerase chain reaction en
dc.subject Sequencing en
dc.subject South Africa (SA) en
dc.subject.lcsh Veterinary medicine -- South Africa
dc.subject.lcsh Virus diseases en
dc.subject.lcsh Bovine viral diarrhoea en
dc.subject.lcsh Bovine viral diarrhoea virus (BVDV) en
dc.title Molecular analysis of bovine viral diarrhoea virus isolates from South Africa en
dc.type Article en


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