Characterization of the interactions of the pneumolysoid, Δ6 PLY, with human neutrophils in vitro
Loading...
Date
Authors
Cockeran, Riana
Theron, Annette J.
Mitchell, Timothy J.
Feldman, Charles
Anderson, Ronald
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
The pneumolysin toxoid, Δ6 PLY, is a prototype pneumococcal protein vaccine candidate. However, its potentially detrimental residual pro-inflammatory interactions with human neutrophils are unknown. In the current study the effects of the toxoid (8–1000 ng/ml) have been compared with those of wild-type pneumolysin (WT/PLY, 8 ng/ml) on neutrophil cytosolic Ca2+ fluxes, generation of leukotriene B4 (LTB4), and release of matrix metalloproteinase-9 (MMP-9), using spectrofluorimetric, and ELISA procedures (LTB4 and MMP-9) respectively. Exposure of neutrophils to WT/PLY resulted in influx of Ca2+ and significant (P < 0.05) release of MMP-9 and generation of LTB4. However, treatment of the cells with Δ6 PLY at concentrations of up to 1000 ng/ml had only trivial effects on Ca2+ influx and no effects on either release of MMP-9 or LTB4 production. The observed absence of pro-inflammatory interactions of Δ6 PLY with neutrophils is clearly an important property of this pneumococcal protein vaccine candidate.
Description
Keywords
Calcium, Leukotriene B4, Matrix metalloproteinase-9, Neutrophils
Sustainable Development Goals
Citation
Cockeran, R, Steel, HC, Theron, AJ, Mitchell, TJ, Feldman, C & Anderson, R, Characterization of the interactions of the pneumolysoid, Δ6 PLY, with human neutrophils in vitro, Vaccine, vol. 29, no.48, pp. 8780–8782 (2011), doi: 10.1016/j.vaccine.2011.09.080