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Browsing Genetics by Subject "T-RFLP"

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Evidence for successional development in Antarctic hypolithic bacterial communities

Makhalanyane, Thulani Peter; Valverde, Angel; Cary, Stephen Craig; Birkeland, Nils-Kare; Tuffin, Marla I.; Cowan, Don A. (Nature Publishing Group, 2013-11)

Hypoliths (cryptic microbial assemblages that develop on the undersides of translucent rocks) are significant contributors to regional C and N budgets in both hot and cold deserts. Previous studies in the Dry Valleys ...
A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches

Gunnigle, Eoin; Ramond, Jean-Baptiste; Frossard, Aline; Seely, Mary; Cowan, Don A. (Elsevier, 2014-08)

A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP ...

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